| 王皓晨,高诚,陶陆阳.脑外伤后蛋白表达变化的蛋白组学研究[J].济宁医学院学报,2017,40(3):157-162 |
| 脑外伤后蛋白表达变化的蛋白组学研究 |
| Proteomics study in acute rat traumatic brain injury model |
| 投稿时间：2017-05-17 |
| DOI：10.3969/j.issn.1000-9760.2017.03.002 |
| 中文关键词: 脑外伤;蛋白组学;生物信息学分析 |
| 英文关键词: Traumatic brain injury;Proteomics;Bioinformatics |
| 基金项目:国家自然科学基金重点项目（81530062） |
| 作者 | 单位 | E-mail | | 王皓晨 | 苏州大学医学部法医学系, 苏州 215021 | | | 高诚 | 苏州大学医学部法医学系, 苏州 215021 | | | 陶陆阳 | 苏州大学医学部法医学系, 苏州 215021 | taoluyang@suda.edu.cn |
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| 中文摘要: |
| 目的 观察SD大鼠在脑外伤急性期的蛋白组学变化，探讨脑外伤后差异蛋白在生物学过程、分子功能以及蛋白-蛋白相互作用中所发挥的作用，为筛选脑外伤分子生物学标记物提供基础数据。方法 8周龄雄性SD大鼠75只，其中每次生物学重复25只共计3次。建立自由落体脑外伤模型，每一次生物学验证设立对照组和损伤组，损伤组又分为伤后1h、6h、24h、3d组，每组5只。按各时间点处死大鼠分离伤侧皮质，提取蛋白并通过胰酶消化成肽段后进行质谱LC-MS/MS检测，通过对肽段信息搜库得到蛋白变化结果并进行生物信息学分析，以上调1.2倍或下调0.8倍表达蛋白作为差异表达蛋白（P<0.05）。结果 在1h、6h、24h、3d 4个时间点中，分别有83、66、127、155个蛋白发生差异变化，并参与多种生物学途径。结论 大鼠脑外伤后蛋白代谢水平发生变化，可在其中筛选出脑外伤的特征性标志物。 |
| 英文摘要: |
| Objective To observe the change of proteins profile and proteomics after TBI (traumatic brain injury) and survey the effect of differentially expressed proteins in biological process,molecular function,cellular component and protein integration.Methods Seventy-five male rats with eight weeks old were divided into five groups including control group and four times point TBI sub-groups (1h,6h,24h,and 3d).The experiment was performed three tines biological repeats.TBI model was established by weight drop device,and the rats were sacrificed at 1h,6h,24h,3d time point after suffering TBI.The brain cortex tissues were undergone LC-MS/MS analysis.Gene ontology (GO) was performed using the bioinformatics analysis tool DAVID.Results After suffering TBI,the proteins with expression ratio of over 1.2-fold in increase or at least 0.8-fold in decrease were considered as significant difference (P<0.05).In four time points,88,66,127 and 155 proteins were significant difference.The varieties of biological pathways were influenced.Conclusion The changes of proteins metabolism after TBI may be strategy for biomarker discovery. |
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