| 刘树玲,丁丽,邢昆.兔组织笼无菌感染模型的制备[J].济宁医学院学报,2015,(1):23-25 |
| 兔组织笼无菌感染模型的制备 |
| The preparation of aseptic rabbit tissue cage infection model |
| 投稿时间：2014-12-26 |
| DOI：10.3969/j.issn.1000-9760.2015.01.005 |
| 中文关键词: 兔组织笼模型;PK-PD;无菌操作;组织液 |
| 英文关键词: A rabbit tissue cage model;PK-PD modeling;Aseptic technique;Tissue fluid |
| 基金项目:2010年山东省自然科学基金项目(编号:ZR2010BL031);2013年济宁医学院基金项目(编号:JY2013KJ011) |
| 作者 | 单位 | | 刘树玲 | 济宁医学院药学院, 山东日照 276826 | | 丁丽 | 济宁医学院药学院, 山东日照 276826 | | 邢昆 | 济宁医学院药学院, 山东日照 276826 |
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| 中文摘要: |
| 目的 制备兔组织笼无菌感染模型,为药物药动学-药效学结合模型(pharmacokinetic-pharmacodynamic modeling,PK-PD)研究提供在体动物实验载体。方法 选取健康家兔28只,耳缘静脉注射盐酸赛拉嗪注射液0.15ml/kg麻醉,每只分别背部备皮、切开皮肤,然后在皮下植入长约5cm的无菌中空胸腔引流管1个,继而在无菌操作下手术缝合。术后应用青霉素肌肉注射3d以预防感染。结果 5只家兔先后因不同原因死亡,23只家兔均获得清亮的无菌组织液,成功建立兔组织模型,成功率为82.14%。结论 通过严格控制无菌操作,选择合适的麻醉剂,手术刀口选择在兔背部,术后及时预防感染,可以成功制备兔组织笼无菌感染模型,并获得清亮的无菌组织液。 |
| 英文摘要: |
| Objective To prepare a rabbit tissue cage model so as to provide drug animal carrier in vivo for drug PK-PD modeling study. Methods 28 healthy rabbits were selected and injected with the anesthesia xylazine iv respectively.The back hair was cut in each rabbit, and then the skin was cut open. A 5cm of sterile hollow chest tube drainage was implanted subcutaneously. In the end the wound was sewed up with aseptic technique. After operation penicillin im was injected for 3 days to prevent infection.Results Among the 28 rabbits, 5 rabbits were dead due to different causes. Clear and sterile tissue fluid were gotten from other 23 rabbits. A rabbit tissue model was established successfully, and the success rate was 82.14%.Conclusion By stricted controlling aseptic operation, selecting the appropriate anesthesia and surgical incision in the back of the rabbit, timely postoperative anti-infection, a rabbit tissue cage model can be successfully established, so does clear tissue fluid. |
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