| 谷颖,金鑫,李霞,沈彩云,石朋.HO-1对AngⅡ诱导大鼠心肌纤维化的影响[J].济宁医学院学报,2019,42(6):395-398 |
| HO-1对AngⅡ诱导大鼠心肌纤维化的影响 |
| Effect of HO-1 on AngⅡ-induced myocardial fibrosis in rats |
| 投稿时间：2019-05-30 |
| DOI：10.3969/j.issn.1000-9760.2019.06.004 |
| 中文关键词: 血红素氧合酶-1;心肌纤维化;心肌成纤维细胞;血管紧张素Ⅱ |
| 英文关键词: Heme oxygenase-1;Myocardial fibrosis;Cardiac fibroblasts;AngiotensinⅡ |
| 基金项目: |
| 作者 | 单位 | E-mail | | 谷颖 | 济宁医学院附属医院, 济宁 272029 | | | 金鑫 | 济宁医学院附属医院, 济宁 272029 | | | 李霞 | 济宁医学院附属医院, 济宁 272029 | | | 沈彩云 | 济宁医学院附属医院, 济宁 272029 | | | 石朋 | 济宁医学院附属医院, 济宁 272029 | bler-8456@163.com |
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| 中文摘要: |
| 目的 探讨血红素氧合酶-1（HO-1）对血管紧张素Ⅱ（AngⅡ）诱导大鼠心肌纤维化的作用。方法 培养Wistar大鼠心肌成纤维细胞（cardiac fibroblasts，CFs），随机分为4组：正常对照组、AngⅡ组（加入AngⅡ）、Hemin组（加入AngⅡ和HO-1诱导剂氯化血红素hemin）和ZnppIX组（加入AngⅡ和HO-1抑制剂锌原卟啉-9 ZnppIX），四甲基偶氮唑盐比色法（MTT）检测各组细胞的增殖率，Western blot检测各组细胞HO-1蛋白的表达。结果 AngⅡ组较正常对照组心肌成纤维细胞增殖率显著升高（P<0.01）；与AngⅡ组比较，Hemin组细胞增殖率显著下降（P<0.01），HO-1蛋白表达水平显著升高（P<0.01）；与AngⅡ组比较，ZnppIX组细胞增殖率无明显变化（P>0.05），HO-1蛋白表达水平降低（P<0.01）。结论 HO-1对AngⅡ诱导的大鼠心肌纤维化具有抑制作用。 |
| 英文摘要: |
| Objective To investigate the effect of heme oxygenase-1 (HO-1) on angiotensin Ⅱ (AngⅡ) -induced myocardial fibrosis in rats.Methods Cultured neonatal Wistar rats' cardiac fibroblasts (CFs) were randomly divided into 4 groups,the normal group,the AngⅡ group (added AngⅡ),the Hemin group (added AngⅡ and hemin which could induce more HO-1) and the ZnppIX group (added AngⅡ and Zinc protoporphyrin-9 which could reduce HO-1).The proliferation of cardiac fibroblasts of each group was detected by MTT assay.The expression of HO-1 protein in cardiac fibroblasts was detected by Western blot.Results The proliferation rate of cardiac fibroblasts in AngⅡ group was significantly higher than that in normal group (P<0.01).The proliferation rate of Hemin group was significantly lower than that of AngⅡ group (P<0.01),while the expression of HO-1 protein was significantly increased (P<0.01).However,there was no significant change in cell proliferation rate in the ZnppIX group compared with the AngⅡ group (P>0.01),while the expression of HO-1 protein was significantly decreased (P<0.01).Conclusion HO-1 could inhibit the AngⅡ-induced myocardial fibrosis in rats. |
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