| 秦燕霞,李京,柳新平.siRNA沉默B7-H4基因对人卵巢癌细胞增殖能力的影响[J].济宁医学院学报,2016,39(5):320-323 |
| siRNA沉默B7-H4基因对人卵巢癌细胞增殖能力的影响 |
| Effect of silencing B7-H4 expression by small interference RNA on proliferation of ovarian carcinoma cells |
| 投稿时间：2016-04-15 |
| DOI：10.3969/j.issn.1000-9760.2016.05.005 |
| 中文关键词: 卵巢癌;B7-H4;siRNA;增殖 |
| 英文关键词: Ovarian carcinoma;B7-H4;siRNA;Proliferation |
| 基金项目:2015年济宁医学院校级科研项目（JY2015KJ018） |
| 作者 | 单位 | | 秦燕霞 | 济宁医学院基础医学院, 济宁 272067 | | 李京 | 济宁医学院基础医学院, 济宁 272067 | | 柳新平 | 济宁医学院基础医学院, 济宁 272067 |
|
| 摘要点击次数: 5460 |
| 全文下载次数: 1064 |
| 中文摘要: |
| 目的 探讨B7-H4基因的小干扰RNA（small interference RNA，siRNA）对卵巢癌SKOV-3细胞生长及增殖的影响。方法 化学合成B7-H4-siRNA，转染SKOV-3细胞，MTT法检测卵巢癌细胞增殖情况，细胞计数法计算细胞倍增时间。结果 B7-H4-siRNA成功转入SKOV-3细胞，B7-H4-siRNA组细胞生长受到明显抑制，细胞倍增时间明显长于未转染组、随机乱码组和转染试剂对照组。结论 B7-H4-siRNA能沉默SKOV-3细胞中B7-H4的表达，抑制SKOV-3细胞的生长增殖，B7-H4可能成为卵巢癌基因治疗的候选靶点。 |
| 英文摘要: |
| Objective To investigate the effect of silencing B7-H4 expression by small interference RNA (siRNA) on proliferation of ovarian carcinoma cells.Methods Chemical synthesized siRNA targeting B7-H4 was transfected into SKOV-3 cells.The proliferation of SKOV-3 cells was determined by MTT assay and cell doubling time was detected by cell counting method.Results B7-H4-siRNA was successfully transfected into SKOV-3 cells.In the B7-H4-siRNA group,the proliferation of SKOV-3 cells was significantly inhibited,and the doubling time was longer than that in the untransfected group,the Ctrl-siRNA group and the transfection reagent group.Conclusion B7-H4-siRNA can silence B7-H4 expression in SKOV-3 cells,and inhibit the proliferation of SKOV-3 cells.B7-H4 can be regarded as a candidate gene for ovarian carcinoma gene therapy. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
