| 吕华,刘培培,胡静飞,李菲,郭娅,陈宗波.OAS2-751C/A位点基因多态性与儿童EV71感染的关联性研究[J].济宁医学院学报,2016,39(2):94-96,100 |
| OAS2-751C/A位点基因多态性与儿童EV71感染的关联性研究 |
| Association of Genetic Polymorphism of OAS2-751C/A with the Susceptibility and Severity of Enterovirus-71 infection in Chinese children |
| 投稿时间：2016-01-20 |
| DOI：10.3969/j.issn.1000-9760.2016.02.005 |
| 中文关键词: 肠道病毒71型;2'-5'寡聚腺苷酸合成酶2;基因多态性 |
| 英文关键词: Enterovirus 71;2'-5'oligadenylate synthetase 2;Polymorphism |
| 基金项目:国家自然科学基金资助项目(311711212) |
| 作者 | 单位 | E-mail | | 吕华 | 青岛大学附属医院, 青岛 266003 | | | 刘培培 | 青岛大学附属医院, 青岛 266003 | | | 胡静飞 | 青岛市妇女儿童医院, 青岛 266003 | | | 李菲 | 青岛大学附属医院, 青岛 266003 | | | 郭娅 | 青岛大学附属医院, 青岛 266003 | | | 陈宗波 | 青岛大学附属医院, 青岛 266003 | drchen001@126.com |
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| 中文摘要: |
| 目的 探讨2'-5'寡聚腺苷酸合成酶2基因5'端调控区751C/A(OAS2-751C/A)位点单核苷酸多态性与肠道病毒71型(EV71)易感性及其病情严重程度的关系。方法 选取2014年6月至2015年9月青岛大学附属医院及青岛市妇女儿童医院收治的EV71检测为阳性的患儿215例,参照《手足口病诊疗指南(第2010版)》的标准,根据病情轻重分为轻症组127例,重症组88例。同期收集无感染症状的健康体检儿童174例,提取外周血DNA,利用多重高温连接酶检测反应(iMLDR)技术检测OAS2-751C/A位点基因多态性。利用SPSS16.0对结果进行统计学分析。结果 OAS2-751C/A位点基因型分布及等位基因频率在EV71感染组及对照组之间差异具有显著统计学意义(P<0.05),EV71感染组A等位基因频率明显高于对照组(P<0.05);轻症组与重症组之间基因型分布及等位基因分布频率无明显差异(P >0.05)。结论 OAS2-751C/A位点A等位基因可能与人体对EV71易感性相关,与病情严重程度无关。A等位基因可能是EV71感染的危险因素。 |
| 英文摘要: |
| Objective The goal of this study was to explore the relationship of the genetic polymorphism of the OAS2-751 with the predisposition and the severity of the enterovirus 71(EV71) infection in children.Methods 215 EV71-infected children were selected in this study whom were admitted in the Affiliated Hospital of Qingdao University and Women and Children hospital of Qingdao from 2014 June to 2015 September.174 healthy children were also selected who received the physical examination in the hospitals at the same time.And the EV71-infected children were divided into 127 mild cases and 88 severe cases according to the Hand,Foot,and Mouth Disease diagnosis and treatment guidelines(2010).Then genomic DNA were extracted from white blood cells.Using an improved multiplex ligation detection reaction (iMLDR) technique the genotype for the C/A polymorphism at the-751 position of OAS2 gene of both groups was detected.The differences among groups were compared by SPSS16.0.Results The genotype distribution and allele frequency of the OAS2-751C/A between the EV71-infected group and healthy control group was significantly different(P<0.05),and the A allele frequency of the EV71-infected group was significant higher than the control group(P<0.05).However,there was no statistically difference in the distribution of the genotype and the allele frequency between the mild and the severe group (P >0.05).Conclusion The A carrier of the OAS2-751C/A was found to be associated with the susceptibility of EV71,but not related to the severity of EV71-infection.And A allele could be the risk factor of EV71 infection in the Chinese children. |
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