| 贾超凡,张凤娇,徐新秋,闫业浩,魏胤,张璟.罗丹明B荧光法检测湖水中铅离子[J].济宁医学院学报,2025,48(2):125-128 |
| 罗丹明B荧光法检测湖水中铅离子 |
| Rhodamine B fluorescence method for detecting lead ions in lake water |
| 投稿时间：2024-11-18 |
| DOI：10.3969/j.issn.1000-9760.2025.02.006 |
| 中文关键词: 罗丹明B;荧光法;铅离子 |
| 英文关键词: Rhodamine B;Fluorescence method;Lead ion |
| 基金项目:山东省自然科学基金青年项目(ZR2020QH297) |
| 作者 | 单位 | E-mail | | 贾超凡 | 滨州医学院公共卫生学院, 烟台 264000
济宁医学院公共卫生学院, 济宁 272013 | | | 张凤娇 | 济宁医学院公共卫生学院, 济宁 272013
山东第一医科大学公共卫生学院, 济南 250000 | | | 徐新秋 | 济宁医学院公共卫生学院, 济宁 272013
山东第一医科大学公共卫生学院, 济南 250000 | | | 闫业浩 | 济宁医学院公共卫生学院, 济宁 272013 | | | 魏胤 | 济宁医学院公共卫生学院, 济宁 272013 | | | 张璟 | 滨州医学院公共卫生学院, 烟台 264000
济宁医学院公共卫生学院, 济宁 272013 | zhangj1976@163.com |
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| 中文摘要: |
| 目的 利用罗丹明B荧光淬灭高灵敏度检测湖水中铅离子(Pb2+)。方法 基于配位作用,罗丹明B与Pb2+结合后,在577 nm处的荧光强度显著降低。通过优化实验条件,记录荧光强度变化,建立荧光强度与Pb2+浓度的线性关系,并评价其在实际样品检测中的准确性和可靠性。结果 Pb2+在45~55 μg/mL浓度范围内与荧光强度呈良好的线性关系,线性方程为y=-25.8761x+3 265.9653,相关系数R2为0.9 746,检出限为1 μg/mL。选择性试验表明,罗丹明B对Pb2+具有较高的选择性,其他常见金属离子(如K+、Mg2+、Mn2+、Na+、Zn2+等)对检测结果的干扰较小。在45 μg/mL和55 μg/mL 2个浓度水平下,平均回收率为93.40%~99.60%,相对标准偏差为0.99%~2.33%。结论 该方法操作便捷,样品处理简单,试剂用量少,选择性好,灵敏度高,能够快速、准确地检测湖水中的Pb2+,具有良好的实际应用潜力。 |
| 英文摘要: |
| Objective To develop a highly sensitive detection method for lead ions (Pb2+) in lake water using Rhodamine B fluorescence quenching. Methods Based on coordination interactions,the fluorescence intensity of Rhodamine B at 577nm significantly decreases upon binding with Pb2+.By optimizing experimental conditions and recording fluorescence intensity changes,a linear relationship between fluorescence intensity and Pb2+ concentration was established.The accuracy and reliability of the method for real sample detection were also evaluated. Results A good linear relationship between Pb2+ concentration and fluorescence intensity was observed in the range of 45～55μg/mL,with a linear equation of y=-25.8761x+3265.9653 and a correlation coefficient R2=0.9746.The detection limit was 1μg/mL.Selectivity tests showed that Rhodamine B exhibited high selectivity for Pb2+,with minimal interference from common metal ions (eg,K+,Mg2+,Mn2+,Na+,Zn2+).At spiked concentrations of 45μg/mL and 55μg/mL,the average recovery rate ranged from 93.40% to 99.60%,with relative standard deviations of 0.99%-2.33%. Conclusion This method is operationally simple,requires minimal sample preparation and reagent usage,and demonstrates excellent selectivity and sensitivity.It enables rapid and accurate detection of Pb2+ in lake water and shows strong potential for practical applications. |
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