| 刘慧娟,吴其国,郭晓军,曹富,余水红.不同产地山药根际土壤微生物群落结构及多糖含量的差异性[J].济宁医学院学报,2024,47(2):83-89 |
| 不同产地山药根际土壤微生物群落结构及多糖含量的差异性 |
| Differences of rhizosphere soil microbial community structure and polysaccharide content of Chinese yam from different origins |
| 投稿时间：2023-07-06 |
| DOI：10.3969/j.issn.1000-9760.2024.02.002 |
| 中文关键词: 山药;根际土壤;微生物群落结构;高通量测序;多糖含量 |
| 英文关键词: Chinese yam;Rhizosphere soil;Microbial community structure;High-throughput sequencing;polysaccharide content |
| 基金项目:安徽省高校自然科学研究项目（KJ2021A1297）；安徽省高校自然科学研究重大项目（2022AH040323） |
| 作者 | 单位 | | 刘慧娟 | 安庆医药高等专科学校药学院, 安庆 246052 | | 吴其国 | 安庆医药高等专科学校药学院, 安庆 246052 | | 郭晓军 | 河北农业大学生命科学学院, 保定 071001 | | 曹富 | 安庆医药高等专科学校药学院, 安庆 246052 | | 余水红 | 安庆医药高等专科学校药学院, 安庆 246052 |
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| 中文摘要: |
| 目的 揭示不同产地山药根际土壤微生物群落结构及多糖含量的差异性。方法 采用高通量测序，对不同产地成熟期山药的根际土壤进行微生物群落结构分析及KEGG、FUNGuild代谢功能预测，同时对山药进行多糖含量测定。结果 放线菌门（Actinobacteriota）、变形菌门（Proteobacteria）和厚壁菌门（Firmicutes）在不同产地样本细菌分类中均占绝对优势。子囊菌门（Ascomycota）、被孢霉门（Mortierellomycota）、担子菌门（Basidiomycota）为优势真菌菌群。河南焦作细菌群落结构的丰富度和多样性指数最高，河北蠡县的真菌群落结构丰富度和多样性指数最高；河北蠡县的细菌以及真菌群落的功能预测丰度均最高，优势菌群是主要的微生物功能预测种群；河北蠡县山药多糖含量最高，湖北武穴次之。结论 山药多糖含量差异性与根际土壤微生物群落结构尤其是优势真菌菌群的代谢功能关联密切。 |
| 英文摘要: |
| Objective To reveal the differences in microbial community structure of rhizosphere soil and polysaccharide content in yam from different origins.Methods High-throughput sequencing was used to analyze the microbial community structure and predict the metabolic functions of KEGG and FUNGuild in the rhizosphere soil of yam at maturity from different origins,while the polysaccharide content of yam was measured.Results Actinobacteriota,Proteobacteria and Firmicutes are absolutely dominant in the bacterial classification of samples from different origins.Ascomycota,Mortierellomycota and Basidiomycota are the dominant fungal groups.Chinese yam in Jiaozuo,Henan province,has the highest abundance and diversity index of bacterial community structure,and the yam in Lixian,Hebei province,have the highest abundance and diversity index of fungal community structure;the yam in Lixian,Hebei province,has the highest functional predicted abundance of both bacterial as well as fungal communities,and the dominant bacterial group is the main microbial functionally predicted group;The yam in Lixian,Hebes province has the highest polysaccharide content,followed by Wuxue,Hubei province.Conclusion Differential analysis of the polysaccharide content of yam showed that it was closely related to the structure of the rhizosphere soil microbial community,especially the metabolic function of the dominant fungal flora. |
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