| 解霜雁,林立,邵华.振动对家兔骨骼肌线粒体呼吸链酶及ATP酶活力的影响[J].济宁医学院学报,2019,42(1):15-18,23 |
| 振动对家兔骨骼肌线粒体呼吸链酶及ATP酶活力的影响 |
| Effects of the vibration on the activities of mitochondrial respiratory chain enzymes and ATPases in the skeletal muscle of rabbits |
| 投稿时间：2018-10-15 |
| DOI：10.3969/j.issn.1000-9760.2019.01.004 |
| 中文关键词: 振动;骨骼肌;线粒体呼吸链酶;ATP酶;能量代谢 |
| 英文关键词: Vibration;Skeletal muscle;Mitochondrial respiratory chain enzyme;ATP enzyme;Energy metabolism |
| 基金项目:国家自然基金项目（81273042） |
| 作者 | 单位 | E-mail | | 解霜雁 | 济南大学山东省医学科学院医学与生命科学学院, 济南 250062
济宁医学院公共卫生学院, 济宁 272067 | | | 林立 | 济宁医学院公共卫生学院, 济宁 272067 | | | 邵华 | 山东省医学科学院山东省职业卫生与职业病防治研究院, 济南 250062 | chinashaohua5888@163.com |
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| 中文摘要: |
| 目的 探讨振动对家兔骨骼肌线粒体呼吸链酶及ATP酶活力的影响及其意义。方法 将32只家兔随机分为低强度组、中强度组、高强度组3个实验组和1个对照组，每组8只。对家兔进行为期45d的振动负荷试验。试验结束后，取其后肢骨骼肌，进行线粒体呼吸链酶复合体Ⅰ、Ⅱ、Ⅲ、Ⅳ及ATP酶活力的测定。结果 对照组、低强度组、中强度组、高强度组骨骼肌组织线粒体呼吸链酶复合体Ⅰ的活力分别是248.30±40.43、180.89±84.67、172.01±40.00、77.87±52.72；线粒体呼吸链酶复合体Ⅱ的活力分别是71.47±5.25、63.91±8.09、52.19±9.21、28.62±5.84；线粒体呼吸链酶复合体Ⅲ的活力分别是31.85±11.62、22.63±8.84、14.26±6.66、7.92±3.35；线粒体呼吸链酶复合体Ⅳ的活力分别是32.76±4.75、12.56±2.91、11.83±1.77、7.11±1.64。与对照组相比，各实验组家兔骨骼肌线粒体呼吸链酶复合体Ⅰ~Ⅳ的活力均明显减弱（P<0.05）。对照组、低强度组、中强度组、高强度组骨骼肌组织中Na+-K+-ATP酶的活力分别是34.23±4.20、15.17±0.68、16.16±0.47、11.56±1.76；Ca2+-Mg2+-ATP酶的活力分别是12.17±1.11、7.15±1.19、5.40±0.92、4.90±1.05。与对照组比较，3个实验组Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活力均明显降低（P<0.05）。结论 振动可导致家兔骨骼肌线粒体呼吸链酶活力降低而引发能量代谢异常，进而可能成为振动性骨骼肌损伤的重要因素。 |
| 英文摘要: |
| Objective To study the effects of vibration on the activities of mitochondrial respiratory chain enzymes and ATPases in the skeletal muscle of rabbits.Methods Thirty-two rabbits were randomly divided into low-intensity group,medium-intensity group,high-intensity group and control group(n=8).The vibration loading test was carried out on each intensity group.After the test,the activities of mitochondrial respiratory chain enzymes and ATPases in the skeletal muscle of each group were measured.Results The activities of mitochondrial respiratory chain enzyme complex Ⅰ in the skeletal muscle tissue of control group,low-intensity group,medium-intensity group and high-intensity group were 248.30±40.43,180.89±84.67,172.01±40.00 and 77.87±52.72;The activities of respiratory chain enzyme complex Ⅱ were 71.47±5.25,63.91±8.09,52.19±9.21 and 28.62±5.84.The activities of respiratory chain enzyme complex Ⅲ were 31.85±11.62,22.63±8.84,14.26±6.66 and 7.92±3.35.Respiratory chain enzyme complex IV were 32.76±4.75,12.56±2.91,11.83±1.77 and 7.11±1.64,respectively.Compared with the control group,the activities of mitochondrial respiratory chain enzyme complexes Ⅰ~Ⅳ in skeletal muscle of each intensity group were significantly decreased (P<0.05).The activities of Na+-K+-ATPase in skeletal muscle tissue of control group,low-intensity group,medium-intensity group and high-intensity group were 34.23±4.20,15.17±0.68,16.16±0.47 and 11.56±1.76,and the activities of Ca2+-Mg2+-ATPase were 12.17±1.11,7.15±1.19,5.40±0.92 and 4.90±1.05,respectively.Compared with the control group,the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in the three intensity groups were significantly decreased (P<0.05).Conclusion The vibration can lead to the decrease of activities of mitochondrial respiratory chain enzymes and abnormal energy metabolism of skeletal muscle in rabbits,which may become an important factor of vibration-induced skeletal musclular injury. |
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