文章摘要
孙珊珊,张千,丁林,刘树玲,刘玉国,李艳芝.蒲公英总黄酮含量测定及对小鼠体液免疫功能的影响[J].济宁医学院学报,2018,41(4):235-237
蒲公英总黄酮含量测定及对小鼠体液免疫功能的影响
Determination of total flavonoids content and effects of Taraxacum mongolicum Hand.Mazz.on humoral immune function in mice
投稿时间:2017-04-18  
DOI:10.3969/j.issn.1000-9760.2018.04.002
中文关键词: 蒲公英;总黄酮;含量测定;体液免疫
英文关键词: Taraxacum mongolicum Hand.Mazz.;Total flavonoids;Content determination;Humoral immunity
基金项目:济宁医学院青年教师科研扶持基金重点项目(JY2016KJ010Z);济宁医学院教师科研扶持基金(JY2017KJ048)
作者单位E-mail
孙珊珊 济宁医学院药学院, 日照 276826  
张千 济宁市食品药品检验检测中心, 济宁 272000  
丁林 济宁医学院药学院, 日照 276826  
刘树玲 济宁医学院药学院, 日照 276826  
刘玉国 济宁医学院药学院, 日照 276826  
李艳芝 济宁医学院药学院, 日照 276826 461328261@qq.com 
摘要点击次数: 662
全文下载次数: 289
中文摘要:
      目的 研究不同采集时间蒲公英(根、叶、花)总黄酮含量变化规律及含量最高时期蒲公英根、叶、花总黄酮对小鼠体液免疫功能的影响。方法 超声提取法提取蒲公英中总黄酮,用HPD-400型大孔树脂进行纯化,紫外分光光度法测定总黄酮含量;溶血素测定法测定小鼠体液免疫功能。结果 蒲公英总黄酮含量4月10日采集的最高。同一时期蒲公英根、叶、花中总黄酮含量高低为花 > 叶 > 根。蒲公英根、叶、花总黄酮均有增强小鼠体液免疫的作用,其中花作用最强,与黄芪精口服液组相比,差异无统计学意义(P>0.05)。结论 不同时期蒲公英根、叶、花中总黄酮含量不同,4月10日采集的蒲公英花含量最高;蒲公英花总黄酮增强小鼠体液免疫的作用最强。
英文摘要:
      Objective To study the changes of total flavonoids in different stages of Taraxacum mongolicum Hand.Mazz.(root,leaf,flower) and the effects of total flavonoids on the humoral immune function in mice.Methods Total flavonoid was extracted by ultrasonic extraction method and purified by HPD-400 macroporous resin.Ultraviolet spectrophotometric method was used for the determination of the content of total flavones of T.mongolicum Hand.Mazz.in different periods,and hemolysin assay method was used for determination of humoral immune function.Results The content of total flavonoids of root,leaf and flower from T.mongolicum Hand.Mazz.were different in different periods.In the same period,the content of total flavonoids from high to low was flower > leaf > root.The total flavonoids of root,leaf and flower from T.mongolicum Hand.Mazz.could enhance humoral immune effect (compared with negative control group,P<0.05).The total flavonoids of flower had the strongest effect in enhancing humoral immune function in mice.Compared with Astragalus essence oral liquid there was not statistically significant (P>0.05).Conclusion The content of total flavonoids of flower from T.mongolicum Hand.Mazz.was collected in April 10 which was much higher than others.The total flavonoids of flower had the strongest effect in enhancing humoral immune function in mice.
查看全文   查看/发表评论  下载PDF阅读器

手机扫一扫看 分享按钮

漂浮通知

  X

近期,网上出现以《济宁医学院学报》杂志社的名义制造的虚假网站,诱骗作者在线投稿,骗取作者版面费或收取中介费,造成恶劣影响。在此提醒广大作者,审慎辨别,谨防上当。 投稿警示:我刊对所有来稿均不收取审稿费。 投稿方式:登录济宁医学院官方网站,在左下方点击“济宁医学院学报”进入《济宁医学院学报》网页,点击作者登录,注册新用户,然后在线投稿。

-->