刘兆明,李超,王萌,张方可,孙珊珊,张春燕,王慧云.桑白皮提取物对酪氨酸酶活性抑制作用研究[J].济宁医学院学报,2017,40(4):264-267 |
桑白皮提取物对酪氨酸酶活性抑制作用研究 |
The inhibitory effect of Cortex Mori extract on tyrosinase activity |
投稿时间:2017-07-12 |
DOI:10.3969/j.issn.1000-9760.2017.04.007 |
中文关键词: 桑白皮;酪氨酸酶;分光光度法;抑制作用;高效液相色谱 |
英文关键词: Cortex Mori;Tyrosinase;Spectrophotometric method;Inhibition;HPLC |
基金项目:济宁医学院大学生创新项目(CX2015054);济宁市科技发展计划项目(2015-57-122);国家大学生创新创业训练计划项目 |
作者 | 单位 | E-mail | 刘兆明 | 济宁医学院药学院, 日照 276826 | | 李超 | 济宁医学院药学院, 日照 276826 | | 王萌 | 济宁医学院药学院, 日照 276826 | | 张方可 | 济宁医学院药学院, 日照 276826 | | 孙珊珊 | 济宁医学院药学院, 日照 276826 | | 张春燕 | 济宁医学院药学院, 日照 276826 | | 王慧云 | 济宁医学院药学院, 日照 276826 | wang_huiyun@126.com |
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中文摘要: |
目的 以左旋多巴为底物考察桑白皮乙醇提取液对马铃薯酪氨酸酶的抑制作用。方法 采用不同极性溶剂对桑白皮有效成分进行提取,比较各种提取液对马铃薯酷氨酸酶的抑制效果;将提取液稀释后考察提取液浓度与抑制作用间的关系,并计算半数抑制浓度IC50值;采用高效液色谱法分析提取液的主要成分,并对具有不同保留时间成分的抑制活性进行考察。结果 甲醇和乙醇两种溶剂提取液的抑制效果最好;桑白皮提取物的抑制作用与其浓度呈现良好相关性,IC50为0.6225 mg/ml;高效液相色谱实验中抑制活性较强成分保留时间主要集中在10~15 min。结论 本研究对从桑白皮中分离纯化一些具有潜在实际应用价值的酪氨酸酶抑制成分提供了有用的参考。 |
英文摘要: |
Objective To investigate the inhibitory effect of ethanol extract from Cortex Mori on potato tyrosinase.Methods Using different polar solvents the effective components were extracted.And the inhibitory effects of the various extracts were compared.The value of IC50 was calculated by correlating the series of extract concentrations and inhibitory effect.The main components of the extract were analyzed by HPLC,and the inhibitory activity of the components with different retention time was investigated.Results Methanol and ethanol extracts showed the best inhibitory effect,and the inhibitory effect showed a good correlation with the extract concentration.The half inhibitory concentration was 0.6225 mg/ml.The potent inhibitory activity components were mainly concentrated in 10~15 min of high performance liquid chromatography experiment.Conclusion This study provides a useful reference for the isolation and purification of some tyrosinase inhibitors with potential practical value from Cortex Mori. |
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