赛春梅,华会明,王建安,王其宝,王慧云.博落回中苯并菲啶类生物碱外消旋体的分离与鉴定[J].济宁医学院学报,2017,40(4):256-263 |
博落回中苯并菲啶类生物碱外消旋体的分离与鉴定 |
Isolation and identification of the racemic benzophenanthridine alkaloids from Macleaya cordata |
投稿时间:2017-07-05 |
DOI:10.3969/j.issn.1000-9760.2017.04.006 |
中文关键词: 博落回;苯并菲啶类生物碱外消旋体;手性拆分 |
英文关键词: Macleaya cordata;Racemicbenzophenanthridine alkaloids;Chiral HPLC separation |
基金项目:国家自然科学基金资助项目(81172958) |
作者 | 单位 | 赛春梅 | 济宁医学院药学院, 日照 276826 | 华会明 | 沈阳药科大学中药学院, 沈阳 110016 | 王建安 | 济宁医学院药学院, 日照 276826 | 王其宝 | 济宁医学院药学院, 日照 276826 | 王慧云 | 济宁医学院药学院, 日照 276826 |
|
摘要点击次数: 3981 |
全文下载次数: 1310 |
中文摘要: |
目的 对博落回[Macleaya cordata(Willd.)R.Br.]中苯并菲啶类生物碱外消旋体进行分离鉴定。方法 采用硅胶柱色谱、氧化铝、ODS、Sephadex LH-20柱色谱和制备高效液相色谱、薄层色谱、手性液相色谱等方法进行分离纯化,通过1D NMR、2D NMR、UV、IR、X-单晶衍射及计算ECD等方法对化合物进行结构鉴定。结果 从博落回乙醇提取物中分离得到11个化合物,分别鉴定为:(+)-博落回新碱A(1),(-)-博落回新碱A(2),(-)-博落回新碱C(3),(+)-博落回新碱C(4),(-)-6-丙酮基二氢血根碱(5),(+)-6-丙酮基二氢血根碱(6),(-)-6-丙酮基二氢白屈菜赤碱(7),(+)-6-丙酮基二氢白屈菜赤碱(8),(±)-6-甲氧基二氢血根碱(9),(±)-6-甲氧基二氢白屈菜赤碱(10),(±)-spallidamine(11)。结论 化合物1~4为新化合物。 |
英文摘要: |
Objective To study the racemic benzophenanthridine alkaloids from Macleaya cordata.Methods Chromatographic methods were used for the isolation and purification,including opening silica gel,aluminium oxide,ODS,Sephadex LH-20 column chromatography,preparative TLC,semi-preparative HPLC,and chiral HPLC separation.Their structures were elucidated on the basis of 1D,2D NMR,UV,IRspectroscopic analyses,single-crystal X-ray diffraction,and electronic circular dichroism calculation.Results Eleven compounds were isolated from the ethanol extract of Macleayacordata.Their structures were identified as(+)-macleayin A (1),(-)-macleayin A (2),(+)-macleayin B (3),(-)-macleayinB (4),(-)-6-acetonyldihydrosanguinarine (5),(+)-6-acetonyldihydrosanguinarine (6),(-)-6-acetonyldihydrochelerythrine (7),(+)-6-acetonyldihydrochelerythrine (8),(±)-6-methoxyldihydrosanguinarine (9),(±)-6-methoxyldihydrochelerythrine (10),and (±)-spallidamine (11).Conclusion Compounds 1~4 were new compounds. |
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|