| 张青青,董冠军,张惠,闫风连,司传平.高盐饮食对内毒素休克小鼠IL-6 IL-12p40表达的影响[J].济宁医学院学报,2017,40(2):106-109 |
| 高盐饮食对内毒素休克小鼠IL-6 IL-12p40表达的影响 |
| Effects of high salt on the expression of IL-6 and IL-12p40 in endotoxic shock mice |
| 投稿时间：2016-12-09 |
| DOI：10.3969/j.issn.1000-9760.2017.02.007 |
| 中文关键词: 高盐;内毒素休克;IL-6;IL-12p40 |
| 英文关键词: High salt;Endotoxic shock;IL-6;IL-12p40 |
| 基金项目: |
| 作者 | 单位 | E-mail | | 张青青 | 济南大学 山东省医学科学院医学与生命科学学院, 济南 250062
济宁医学院免疫学与分子医学研究所, 济宁 272067 | | | 董冠军 | 济宁医学院免疫学与分子医学研究所, 济宁 272067 | | | 张惠 | 济宁医学院免疫学与分子医学研究所, 济宁 272067 | | | 闫风连 | 济宁医学院免疫学与分子医学研究所, 济宁 272067 | | | 司传平 | 济宁医学院免疫学与分子医学研究所, 济宁 272067 | chpsi@163.com |
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| 中文摘要: |
| 目的 观察高盐饮食对小鼠内毒素休克致死率及炎症因子IL-6和IL-12p40表达的影响。方法 雌性C57BL/6小鼠，6~8周，随机分为2组，每组各10只，高盐饮食组饮用含2.5% NaCl盐水；正常饮食组饮用不含NaCl的清水，喂食14d。腹腔注射脂多糖（LPS，500 μg/只），观察小鼠的死亡率；腹腔注射LPS（200 μg/只），观察小鼠血清、脾脏和腹腔细胞中细胞因子IL-6和IL-12p40水平。结果 1）高盐饮食组小鼠死亡率明显高于正常饮食组；2）与正常饮食组相比，高盐饮食组血清中IL-6和IL-12p40含量明显升高，差异具有统计学意义（t=5.534，P<0.01；t=2.906，P<0.05）；3）与正常饮食组相比，高盐饮食组脾脏细胞中IL-6和IL-12p40 mRNA水平明显升高，差异具有统计学意义（t=4.075，P<0.05；t=4.758，P<0.01），腹腔细胞中IL-6和IL-12p40 mRNA水平明显升高，差异具有统计学意义（t=25.47，P<0.001；t=8.589，P<0.001）。结论 高盐饮食可增加内毒素休克小鼠的死亡率及其炎症因子IL-6和IL-12p40的分泌。 |
| 英文摘要: |
| Objective To observe the effect of high salt on mortality of endotoxic shock mice and the expression of inflammatory factors IL-6 and IL-12p40.Methods Twenty female mice were randomly divided into normal control group and high salt diets group.Respectively,the high salt group was fed with drink containing 2.5% NaCl and the normal control group was given water without NaCl.After 14 days,each mice was injected 500μg LPS to observe mortality.And each mice was injected 200μg LPS to observe the expression of IL-6 and IL-12p40 in serum,spleen and peritoneal cell.Results 1) The mortality of high salt diet mice was obviously higher than normal diet group.2) Compared with normal control group,the serum IL-6 and IL-12p40 were significantly increased in high salt diet mice,and the difference was statistically significant (t=5.534,P<0.01;t=2.906,P<0.05).3) Compared with normal control group,the expression of IL-6 and IL-12p40 in spleen was significantly increased in high salt diet group,and the difference was statistically significant (t=4.075,P<0.05;t=4.758,P<0.01),The expression of IL-6 and IL-12p40 in peritoneal cells was significantly increased in high salt diet group,and the difference was statistically significant (t=25.47,P<0.001;t=8.589,P<0.001).Conclusion High salt diet increased the mortality of endotoxic shock mice and increased the secretion of inflammatory cytokines IL-6 and IL-12p40. |
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