| 马允允,梁秋华,张正军,孟强,李懂,孙琳.长春西汀对小鼠血管平滑肌细胞成骨样分化的影响[J].济宁医学院学报,2016,39(2):82-86 |
| 长春西汀对小鼠血管平滑肌细胞成骨样分化的影响 |
| Effect of vinpocetine on the osteoblastic differentiation of mouse vascular smooth muscle cells |
| 投稿时间：2015-02-09 |
| DOI：10.3969/j.issn.1000-9760.2016.02.002 |
| 中文关键词: 动脉钙化;血管平滑肌细胞;成骨样分化;长春西汀 |
| 英文关键词: Vascular calcification;Vascular smooth muscle cells;Osteoblastic differentiation;Vinpocetine |
| 基金项目:国家自然科学基金资助项目(81400860) |
| 作者 | 单位 | E-mail | | 马允允 | 济宁医学院, 济宁 272067 | | | 梁秋华 | 济宁医学院附属医院, 济宁 272029 | | | 张正军 | 济宁医学院附属医院, 济宁 272029 | | | 孟强 | 济宁医学院附属医院, 济宁 272029 | | | 李懂 | 济宁医学院附属医院, 济宁 272029 | | | 孙琳 | 济宁医学院附属医院, 济宁 272029 | sunlinsddx@sina.com |
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| 中文摘要: |
| 目的 初步探讨长春西汀对小鼠血管平滑肌细胞(vascular smooth muscle cells,VSMCs)成骨样分化的影响。方法 使用10mMβ-甘油磷酸钠(beta-glycerophosphate,β-GP)诱导小鼠原代VSMCs向成骨样分化,以10μM长春西汀干预β-GP诱导的小鼠VSMCs,应用Western blotting分析法检测Runt相关转录因子2(Run related transcription factor 2,Runx2),骨形成蛋白-2(bone morphology protein-2,BMP-2)以及核转录因子-Kappa B(nuclear factor kappaB,NF-κB)p65的表达,碱性磷酸酶(alkaline phosphatase,ALP)试剂盒检测ALP活性,茜素红染色观察矿化结节形成的情况。结果 1)β-GP可显著增强ALP的活性,增加Runx2和BMP-2的表达,并促进矿化结节的形成;2)长春西汀可显著减弱ALP活性,抑制Runx2和BMP-2的表达,减少矿化结节的形成;3)长春西汀抑制NF-κB活化。结论 长春西汀可通过NF-κB信号通路显著抑制小鼠VSMCs成骨样分化。 |
| 英文摘要: |
| Objective To investigate the effect of vinpocetine on the osteoblastic differentiation of mouse vascular smooth muscle cells (VSMCs).Methods 10mM of beta-glycerophosphate (β-GP) was used to induce osteoblastic differentiation of mouse VSMCs.10μM of vinpocetine was treated on the β-GP-stimulated VSMCs.The protein expression of Run related transcription factor 2 (Runx2),bone morphology protein-2 (BMP-2) and nuclear factor kappaB (NF-κB) p65 subunit were determined by Western Blot.Alkaline phosphatase (ALP) assay kit was used to determine the ALP activity.The formation of mineralized nodules was determined by Alizarin Red S staining.Results 1) β-GP significantly increased ALP activity and the expression of Runx2 and BMP-2 which promoted the formation of mineralized nodules.2) Vinpocetine significantly decreased ALP activity and the expression of Runx2 and BMP-2,which attenuated the formation of mineralized nodule.3) Vinpocetine significantly inhibited the translocation of NF-κB p65 into the nucleus.Conclusion Vinpocetine may exert its inhibitory effect on osteoblastic differentiation of VSMCs via NF-κB signaling pathway. |
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