| 王友,朱苏红,王建礼,林娜,王保生.利多卡因后处理对家兔心肌缺血-再灌注损伤TNF-α NF-κB表达的影响[J].济宁医学院学报,2015,(5):318-320 |
| 利多卡因后处理对家兔心肌缺血-再灌注损伤TNF-α NF-κB表达的影响 |
| The effect of lidocaine post-treatment on TNF-α and NF-κB in rabbits with myocardial ischemia reperfusion injury |
| 投稿时间：2015-08-17 |
| DOI：10.3969/j.issn.1000-9760.2015.05.004 |
| 中文关键词: 利多卡因|后处理|心肌缺血-再灌注损伤|NF-κB|TNF-α |
| 英文关键词: Lidocaine|Post-treatment|Myocardial ischemia reperfusion|NF-κB|TNF-α |
| 基金项目:2008年济宁医学院青年基金资助项目 |
| 作者 | 单位 | | 王友 | 济宁医学院基础学院, 山东济宁, 272067 | | 朱苏红 | 济宁医学院基础学院, 山东济宁, 272067 | | 王建礼 | 济宁医学院基础学院, 山东济宁, 272067 | | 林娜 | 济宁医学院基础学院, 山东济宁, 272067 | | 王保生 | 济宁医学院基础学院, 山东济宁, 272067 |
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| 中文摘要: |
| 目的 研究家兔心肌缺血-再灌注损伤时,利多卡因后处理对其体内TNF-α水平及NF-κB表达的影响。方法 18只家兔随机分为假手术组、缺血-再灌注组和利多卡因组3组。结扎家兔心脏的左室支40min后解除结扎,恢复血液供应,再灌注2h后测定各组家兔血浆TNF-α的水平,通过免疫组化染色和免疫印迹方法观察心肌NF-κB的表达情况。结果 与缺血-再灌注组比较,利多卡因组再灌注后血浆TNF-α浓度明显降低,心肌细胞NF-κB的表达显著减少。结论 利多卡因可以抑制再灌注心肌炎症反应,减轻损伤。 |
| 英文摘要: |
| Objective To investigate the protective effects of lidocaine post-treatment on TNF-α and NF-κB in rabbits with myocardial ischemia reperfusion injury.Methods Eighteen rabbits were randomly divided into 3 groups,sham operation group,ischemic reperfusion group and lidocaine group.The left ventricular branch (LVB) of rabbits was ligated for 40minutes,then the ligation was removed.Afer 120 minutes the level of plasma TNF-α was measured,meanwhile the degree of NF-κB was detected with immumohistochemical staining and Western blot.Results Compared with those of ischemic reperfusion group,the concentration of TNF-αwas decreased in lidocaine group (P<0.01).The expression of NF-κB was inhibited by lidocaine too.Conclusion The results demonstrated that lidocaine could protect cardiac function,inhibit inflammatory response and attenuate reperfusion injury in ischemic heart. |
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