| 李亚男,初义超,闫业浩,刘寒,刘克颜,刘慧.基于丙二腈衍生物的SO2新型荧光探针的制备及应用[J].济宁医学院学报,2026,49(1):31-36 |
| 基于丙二腈衍生物的SO2新型荧光探针的制备及应用 |
| Preparation and application of a novel fluorescent probe for SO2 based on malononitrile derivatives |
| 投稿时间：2025-09-16 |
| DOI：10.3969/j.issn.1000-9760.2026.01.006 |
| 中文关键词: 丙二腈衍生物;荧光探针;亚硫酸盐;环境检测 |
| 英文关键词: Malononitrile derivatives;Fluorescent probe;Sulfite;Environmental monitoring |
| 基金项目: |
| 作者 | 单位 | E-mail | | 李亚男 | 济宁医学院公共卫生学院, 济宁 272013 | | | 初义超 | 长春中医药大学临床医学院, 长春 130117 | | | 闫业浩 | 济宁医学院公共卫生学院, 济宁 272013 | | | 刘寒 | 济宁医学院公共卫生学院, 济宁 272013 | | | 刘克颜 | 济宁医学院公共卫生学院, 济宁 272013 | | | 刘慧 | 济宁医学院公共卫生学院, 济宁 272013 | 1603892475@qq.com |
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| 中文摘要: |
| 目的 设计并合成一种基于丙二腈衍生物的新型SO2荧光探针YMC-1，用于检测环境水样中的HSO3-/SO32-，为环境污染监测提供高效检测方法。方法 通过丙二腈与4-二乙氨基苯甲醛反应合成探针YMC-1，利用滴定实验、选择性实验、抗干扰性实验验证其对HSO3-/SO32-的特异性响应，并采用实际水样(济南黄河水和大明湖湖水)及试纸条进行验证。结果 在PBS缓冲液(pH=7.4)中，YMC-1在650 nm处具有最强荧光发射，加入HSO3-/SO32-后荧光显著猝灭，在6~18 μM范围内呈良好线性关系(R2=0.99)，检测限(LOD)为183 nM；紫外吸收在610 nm处随HSO3-/SO32-浓度增加而降低，8~20 μM范围内线性良好(R2=0.99)。探针对多种常见离子和生物硫醇具有良好抗干扰能力，反应在28 min内达到平衡。黄河水和大明湖湖水加标回收率为95.46%~114.52%；所制备试纸条于可见光与紫外光下均呈现明显颜色变化，可实现直观检测。结论 YMC-1探针具有高灵敏性、高选择性和快速响应特性，适用于实际水样中HSO3-/SO32-的检测。 |
| 英文摘要: |
| Objective To design and synthesize a novel fluorescent probe YMC-1 based on malononitrile derivatives for the detection of HSO3-/SO32- in environmental water samples, providing an efficient method for environmental pollution monitoring. Methods The probe YMC-1 was synthesized by reacting malononitrile with 4-(diethylamino) benzaldehyde.Its specific response to HSO3-/SO32- was verified by titration experiments, selectivity experiments, and anti-interference experiments.The probe was further validated using real water samples (water from the Yellow River and Daming Lake) and test strips. Results In PBS buffer (pH=7.4), YMC-1 exhibited the strongest fluorescence emission at 650 nm, which significantly quenched upon addition of HSO3-/SO32-.A good linear relationship was observed in the concentration range of 6~18 μM (R2=0.99) with a detection limit of 183 nM.The ultraviolet absorbance at 610 nm decreased with increasing HSO3-/SO32- concentration, showing a good linear relationship in the range of 8~20 μM (R2=0.99).The probe demonstrated good anti-interference ability against various common ions and biological thiols, and the reaction reached equilibrium within 28 minutes.The spiked recovery rates for real water samples ranged from 95.46% to 114.52%.The prepared test strips showed obvious color changes under both visible light and ultraviolet light, enabling visual detection. Conclusion The YMC-1 probe exhibits high sensitivity, high selectivity, and rapid response, which is suitable for the detection of HSO3-/SO32- in real water samples. |
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