| 王善鹏,杨翠媛,孙金鹏,于斌,王荣,孙闵.RGS22在泛癌中的表达及与预后、免疫浸润的相关性[J].济宁医学院学报,2025,48(6):530-538 |
| RGS22在泛癌中的表达及与预后、免疫浸润的相关性 |
| Expression of RGS22 in pan-cancer and its correlation analysis with prognosis and immune infiltration |
| 投稿时间：2025-07-19 |
| DOI：10.3969/j.issn.1000-9760.2025.06.009 |
| 中文关键词: RGS22;泛癌;免疫浸润;肿瘤微环境 |
| 英文关键词: RGS22;Pan-cancer;Immune infiltration;Tumor microenvironment |
| 基金项目:山东省中医药重点学科方剂学(20221012);山东省中医药科技项目(M20245103);济宁医学院大学生创新训练计划项目(cx2024230z) |
| 作者 | 单位 | E-mail | | 王善鹏 | 济宁医学院中西医结合学院, 济宁 272067 | | | 杨翠媛 | 济宁医学院中西医结合学院, 济宁 272067 | | | 孙金鹏 | 济宁医学院中西医结合学院, 济宁 272067 | | | 于斌 | 济宁医学院中西医结合学院, 济宁 272067 | | | 王荣 | 济宁医学院中西医结合学院, 济宁 272067 | | | 孙闵 | 济宁医学院中西医结合学院, 济宁 272067 | sunmin0219@163.com |
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| 中文摘要: |
| 目的 探究RGS22在泛癌中的表达与预后、肿瘤微环境(TME)及肿瘤免疫浸润之间的关系。方法 利用TIMER、UALCAN、KM plotter、TISIDB和相关R软件包,分析在泛癌中RGS22基因的差异表达,以及与预后、免疫亚型、分子亚型、免疫细胞和免疫检查点(ICPs)的关系,并收集临床样本进行初步验证。结果 RGS22在大多数肿瘤中低表达(P＜0.001);8种肿瘤中RGS22高表达、8种肿瘤中RGS22低表达与较好的预后相关(P＜0.05);7种肿瘤的免疫亚型、10种肿瘤的分子亚型与RGS22的表达显著相关(P＜0.05);在大多数肿瘤中RGS22的表达与肿瘤微环境(TME)中CD4+T细胞和CD8+T细胞等6种免疫细胞浸润水平显著相关(P＜0.05);29种肿瘤中RGS22的表达与10种免疫检查点(ICPs)的表达显著相关(P＜0.05);16种肿瘤中RGS22与肿瘤突变负荷(TMB)显著相关、10种肿瘤中RGS22与微卫星不稳定性(MSI)显著相关(P＜0.05);免疫组化(IHC)初步结果表明,肝细胞癌(LIHC)组织中RGS22、CD4及CD8表达较癌旁组织显著降低,且呈正相关(r=0.839、0.835,P＜0.01)。结论 RGS22在泛癌中的表达与肿瘤的预后及免疫浸润有关,可能是一种潜在的肿瘤预测及预后的生物标志物。 |
| 英文摘要: |
| Objective To investigate the associations between RGS22 expression,prognosis,tumor microenvironment and tumor immune infiltration in pan-cancer. Methods Databases and tools including TIMER,UALCAN,KM plotter,TISIDB,and relevant R packages were utilized to analyze the differential expression of the RGS22 gene across pan-cancers and its associations with prognosis,immune/molecular subtypes,immune cells,and immune checkpoints (ICPs).Clinical samples were collected for preliminary validation. Results RGS22 was lowly expressed in most tumors (P＜0.001).High expression of RGS22 in 8 tumor types and low expression of RGS22 in 8 tumor types were both correlated with better prognosis (P＜0.05).Immune subtypes of 7 tumors and molecular subtypes of 10 tumors were significantly correlated with RGS22 expression (P＜0.05).In most tumors,RGS22 expression significantly correlates with the infiltration levels of 6 immune cell types,including CD4+ T cells and CD8+ T cells,within the TME (P＜0.05).RGS22 expression significantly correlates with the expression of 10 ICPs in 29 tumor types (P＜0.05).RGS22 was significantly correlated with Tumor Mutational Burden (TMB) in 16 tumors and with Microsatellite Instability (MSI) in 10 tumors (P＜0.05).Preliminary immunohistochemistry (IHC) results showed that the expression of RGS22,CD4,and CD8 in Hepatocellular Carcinoma (LIHC) tissues were significantly lower than those in paraneoplastic tissues and were positively correlated (r=0.839,0.835,P＜0.01). Conclusion The expression of RGS22 in pan-cancer is associated with tumor prognosis and immune infiltration,and it may serve as a potential biomarker for tumor prediction and prognosis. |
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