| 朱世军,陈莎莎,黄洪贞,胡堂彬,刘庆胜,聂志奎,张茂枢.掺锶抗生素骨水泥对成骨细胞增殖活性及抗菌性能的影响[J].济宁医学院学报,2025,48(4):304-308 |
| 掺锶抗生素骨水泥对成骨细胞增殖活性及抗菌性能的影响 |
| Effects of strontium-doped antibiotic bone cement on osteoblasts proliferation and antibacterial properties |
| 投稿时间：2024-11-14 |
| DOI：10.3969/j.issn.1000-9760.2025.04.004 |
| 中文关键词: 锶;抗生素骨水泥;成骨细胞;细胞增殖;抑菌能力 |
| 英文关键词: Strontium;Antibiotic bone cement;Osteoblast;Cell proliferation;Antibacterial ability |
| 基金项目:山东省中医药科技项目(M-2023048) |
| 作者 | 单位 | E-mail | | 朱世军 | 济宁市第一人民医院骨关节外科 | | | 陈莎莎 | 济宁市第一人民医院心内科, 济宁 272000 | | | 黄洪贞 | 济宁市第一人民医院骨关节外科 | | | 胡堂彬 | 济宁市第一人民医院骨关节外科 | | | 刘庆胜 | 济宁市第一人民医院骨关节外科 | | | 聂志奎 | 济宁市第一人民医院骨关节外科 | | | 张茂枢 | 济宁市第一人民医院骨关节外科 | zmskmyxy@126.com |
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| 中文摘要: |
| 目的 探讨掺锶抗生素骨水泥(AIBC-Sr)对成骨细胞增殖及抗菌性能的影响。方法 以六水氯化锶(SrCl2·6H2O)掺入抗生素骨水泥(AIBC)中制备AIBC-Sr,掺锶比例分别为0.1%、0.5%、1%、2%,各组分别为AIBC-Sr0.1组、AIBC-Sr0.5组、AIBC-Sr1组、AIBC-Sr2组,未掺锶为对照组。微机控制电子万能试验机测试各组骨水泥抗压强度,接触法测试骨水泥面团时间,热成像仪测试骨水泥固化时间、固化温度和最高聚合温度。根据上述各组不同比例AIBC-Sr材料制备片剂,然后用片剂制备浸提液,未掺锶AIBC浸提液为对照组,依照掺锶比例分为AIBC-Sr0.1、AIBC-Sr0.5、AIBC-Sr1及AIBC-Sr2各浸提液组。各组浸提液培养小鼠颅顶前成骨细胞(MC3T3-E1细胞),细胞增殖-毒性检测试剂盒(CCK-8)检测细胞增殖活性。各组浸提液进行金黄色葡萄球菌、大肠埃希菌抑菌实验,检测抑菌圈直径。结果 AIBC不同比例掺锶后,与对照组相比抗压强度、面团时间、固化时间、最高聚合温度及固化温度均无明显差异(P＞0.05)。CCK-8结果显示,与对照组相比,AIBC-Sr0.5浸提液组细胞增殖活性增高(tD=3.18,P=0.03),差异有统计学意义。对金黄色葡萄球菌抑菌实验中,与对照组比较,AIBC-Sr0.1浸提液组、AIBC-Sr0.5浸提液组、AIBC-Sr1浸提液组和AIBC-Sr2浸提液组抑菌圈直径增大(tD=4.89,P＜0.001;tD=10.20,P＜0.001;tD=4.68,P＜0.001;tD=9.05,P＜0.001),差异均有统计学意义。对大肠埃希菌抑菌实验中,与对照组比较,AIBC-Sr0.1浸提液组、AIBC-Sr0.5浸提液组、AIBC-Sr1浸提液组和AIBC-Sr2浸提液组抑菌圈直径增大(tD=3.17,P=0.03;tD=12.04,P＜0.001;tD=6.34,P＜0.001;tD=13.95,P＜0.001),差异均有统计学意义。结论 AIBC-Sr能够促进MC3T3-E1细胞增殖,增强AIBC对金黄色葡萄球菌、大肠埃希菌的抑菌能力。 |
| 英文摘要: |
| Objective To investigate the effect of strontium-doped antibiotic bone cement on osteoblast proliferation and antibacterial properties. Methods AIBC-Sr was prepared by adding strontium chloride hexahydrate (SrCl2·6H2O) into antibiotic bone cement (AIBC).The proportion of strontium was 0.1%,0.5%,1% and 2%,and the groups were AIBC-Sr0.1,AIBC-Sr0.5,AIBC-Sr1,AIBC-Sr2,respectively.A non-doped AIBC group served as the control.The compression strength of each group of bone cement was tested by micro-controlled electronic universal testing machine,the dough time of bone cement was tested by contact method,and the curing time,curing temperature and maximum polymerization temperature of bone cement were tested by thermal imager.Extract solutions of AIBC-Sr tablets in different proportions were prepared.The extract solution of AIBC without strontium doping was the control group and was divided into the extract solution groups of AIBC-Sr0.1,AIBC-Sr0.5,AIBC-Sr1 and AIBC-Sr2 according to the strontium doping ratio.Mouse cranial precranial osteoblasts (MC3T3-E1 cells) were cultured in the extracts of each group,and cell proliferation-toxicity assay kit (CCK-8) was used to detect cell proliferation activity.Control group,0.1%,0.5%,1% and 2% AIBC-Sr extract groups were selected for staphylococcus aureus and escherichia coli antibacterial experiment,and the diameter of antibacterial circle was detected. Results After AIBC was doped with strontium in different proportions,there were no significant differences in compressive strength,dough time,curing time,maximum polymerization temperature and curing temperature compared with the control group (P＞0.05).The CCK-8 results showed that compared with the control group,the proliferation activity in the AIBC-Sr0.5 extract solution group increased (tD=3.18,P=0.03),and the difference was statistically significant.In the antibacterial experiment against staphylococcus aureus,compared with the control group,the diameters of the antibacterial zones in the AIBC-Sr0.1, AIBC-Sr0.5, AIBC-Sr1 and AIBC-Sr2 extract solution groups increased (tD=4.89,P＜0.001);tD=10.20,P＜0.001;tD=4.68,P＜0.001;tD=9.05,P＜0.001),and the differences were statistically significant.In the antibacterial experiment against escherichia coli,compared with the control group,the diameters of the antibacterial zones in the AIBC-Sr0.1,AIBC-Sr0.5,AIBC-Sr1 and AIBC-Sr2 extract solution groups increased (tD=3.17,P=0.03);tD=12.04,P＜0.001;tD=6.34,P＜0.001;tD=13.95,P＜0.001),and the differences were statistically significant. Conclusion AIBC-Sr extract can significantly promote the proliferation of MC3T3-E1 cells,and enhance the antibacterial efficacy of AIBC against staphylococcus aureus and escherichia coli. |
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