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| 基于丙二腈衍生物的SO2新型荧光探针的制备及应用 |
| Preparation and Application of a Novel Fluorescent Probe for SO2 Based on Malononitrile Derivatives |
| 投稿时间：2025-01-15 修订日期：2026-01-14 |
| DOI： |
| 中文关键词: 丙二腈衍生物，荧光探针，HSO3-/SO32- |
| 英文关键词: Malonitrile derivatives, fluorescence probes，HSO3-/SO32- |
| 基金项目: |
| 作者 | 单位 | 邮编 | | 李亚男 | 济宁医学院 | 272000 | | 初义超 | 长春中医药大学 | | | 闫业浩 | 济宁医学院 | | | 刘寒 | 济宁医学院 | | | 刘克颜 | 济宁医学院 | | | 刘慧* | 济宁医学院 | 272000 |
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| 中文摘要: |
| 目的：设计并合成一种基于丙二腈衍生物的新型SO?荧光探针YMC-1。该探针用于检测环境水样中的HSO3-/SO32-，为环境污染监测提供一种高效检测方法。方法：通过丙二腈与4-二乙氨基苯甲醛反应合成YMC-1探针，利用滴定实验、选择性实验、抗干扰性实验等验证了该探针对于HSO3-/SO32-的特异性响应，并利用实际水样（黄河水和大明湖湖水）以及试纸条进行了验证。结果：荧光响应：在 PBS 缓冲液（pH=7.4）中，YMC-1探针在650 nm处有最强荧光发射，加入 HSO??/SO?2?后荧光强度显著猝灭，在 6~18 μM 浓度区间内与 HSO??/SO?2?浓度呈良好线性关系（R2=0.99，线性方程y=-37.78x+1196.47），检测限为183 nM；紫外响应：YMC-1探针在 610 nm 处有最大吸光度，随HSO??/SO?2?浓度增加吸光度降低，8~20 μM浓度区间线性关系良好（R2=0.99，线性方程 y=-0.04x+1.35）。且对多种常见离子和生物硫醇具有良好的抗干扰能力。加入 HSO??/SO?2?后，28分钟内反应达平衡。YMC-1探针在实际水样（济南黄河水和大明湖湖水）检测中的加标回收率为95.26%-114.52%，符合定量检测要求；所制备的试纸条在18分钟内可见光和紫外光下均对HSO3-/SO32-表现出特殊的颜色变化，可实现直观检测。结论：YMC-1探针对HSO3-/SO32-的检测具有高灵敏性、高选择性和直观检测，可适用于实际水样监测。 |
| 英文摘要: |
| Abstract
Objective: A new SO? fluorescent probe named YMC-1 was designed and synthesized using malononitrile derivatives to detect HSO??/SO?2? in environmental water samples, offering an effective approach for environmental pollution surveillance.
Methods: The YMC-1 probe was synthesized by reacting malononitrile with 4- (diethylamino)benzaldehyde.Its particular response to HSO??/SO?2? was confirmed through titration experiments, selectivity experiments, anti-interference experiments and other relevant tests. Moreover, the probe was tested with real water samples (water from the Yellow River and Daming Lake) and test strips.
Results:Within the PBS buffer (pH = 7.4), the YMC-1 probe demonstrated the most intense fluorescence emission at 650 nm and addition of HSO??/SO?2? ,the fluorescence intensity was highly quenched.A fine linear connection was noticed within the concentration range of 6 to 18 μM (R2 = 0.99, linear equation: y = -37.78x + 1196.47), and there was a limit of detection (LOD) of 183 nM. UV response: The YMC - 1 probe had the greatest absorbance at 610 nm, and as the concentration of HSO??/SO?2? increased, the absorbance decreased;A rather good linear association was attained within the concentration range of 8 to 20 μM (R2 = 0.99, linear equation: y = -0.04x + 1.35),and the LOD was the same as that of the fluorescent detection. The probe had remarkable anti-interference capability regarding various ordinary ions and biological thiols.After HSO??/SO?2? was added, the reaction attained equilibrium within 28 minutes.When detecting real water samples (Yellow River water and Daming Lake water in Jinan), the spiked recovery rate of the YMC - 1 probe varied between 95.26% and 114.52%, satisfying the demands of quantitative detection.The prepared test strips exhibited evident color alterations under both visible light and 365 nm ultraviolet light in response to HSO??/SO?2? within 18 minutes, making visual detection possible.
Conclusion: The YMC-1 probe has the traits of being highly sensitive, highly selective, and having a fast response when it comes to detecting HSO??/SO?2?, which made it appropriate for monitoring real water samples.
Keywords: Malononitrile derivatives; Fluorescent probes; HSO??/SO?2?. |
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