| 姜丽媛,魏元,夏岩,赵琦玥.牙髓干细胞来源外泌体减轻牙周炎和促进牙周组织再生的作用[J].济宁医学院学报,2024,47(6):466-470 |
| 牙髓干细胞来源外泌体减轻牙周炎和促进牙周组织再生的作用 |
| The effect of extracellular vesicles derived from dental pulp stem cells on alleviating periodontitis and promoting periodontal tissue regeneration |
| 投稿时间：2024-05-06 |
| DOI：10.3969/j.issn.1000-9760.2024.06.002 |
| 中文关键词: 牙髓干细胞;外泌体;牙周炎;牙周组织再生 |
| 英文关键词: Dental pulp stem cells;Exosome;Periodontitis;Periodontal tissue regeneration |
| 基金项目:安徽省卫生健康科研项目(AHWJ20226035) |
| 作者 | 单位 | | 姜丽媛 | 安徽医学高等专科学校口腔医学院(附属口腔医院), 合肥 230601 | | 魏元 | 安徽医学高等专科学校口腔医学院(附属口腔医院), 合肥 230601 | | 夏岩 | 安徽医学高等专科学校口腔医学院(附属口腔医院), 合肥 230601 | | 赵琦玥 | 安徽医学高等专科学校口腔医学院(附属口腔医院), 合肥 230601 |
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| 中文摘要: |
| 目的 分析牙髓干细胞(DPSC)来源外泌体(Exos)减轻牙周炎和促进牙周组织再生的作用。方法 选择30只SPF级SD大鼠,分为对照组、模型组、实验组,其中对照组不给予处理,模型组、实验组建立慢性牙周炎(CP)大鼠模型,每组各10只大鼠。实验组选择上颌双侧第一磨牙腭侧牙龈给予DPSC-Exos注射。比较3组大鼠釉牙骨质界至牙槽嵴顶(CEJ-ABC)距离及牙周组织变化。培养牙周膜干细胞(PDLSCs),分为对照组、实验组,对照组不给予处理,实验组培养皿中加入DPSC-Exos,比较两组细胞的迁移能力、增殖能力、钙结节数量,检测两组细胞Runt相关转录因子-2(Runx-2) mRNA、骨涎蛋白(BSP)mRNA及蛋白表达情况。结果 对照组大鼠牙周组织未见异常情况;模型组大鼠牙周组织可见胶原纤维排列紊乱,出现大量炎症细胞浸润,袋内上皮增生明显且呈向根方偏移的情况,同时存在牙槽骨吸收情况;实验组大鼠牙周组织炎症细胞浸润较模型组减轻,龈沟上皮未见明显增生及向根方偏移的情况。模型组大鼠CEJ-ASC距离相较于对照组明显升高;实验组大鼠CEJ-ASC距离相较于模型组明显降低(P＜0.05)。24h、72h时点时,实验组细胞OD值相较于对照组明显升高;12h、24h时点中,实验组细胞迁移率相较于对照组明显升高(P＜0.05)。实验组细胞钙结节数量相较于对照组明显升高(P＜0.05)。实验组细胞Runx-2 mRNA、BSP mRNA、Runx-2蛋白、BSP蛋白相较于对照组明显升高(P＜0.05)。结论 DPSC-Exos可减轻牙周炎大鼠炎症症状,降低骨吸收程度,同时DPSC-Exos可促进PDLSCs细胞的增殖及迁移活动,增强PDLSCs细胞的成骨分化能力,其机制可能与其介导Runx-2、BSP表达有关。 |
| 英文摘要: |
| Objective To analyse the role of dental pulp stem cell (DPSC)-derived exosomes (Exos) in alleviating periodontitis and promoting periodontal tissue regeneration. Methods Thirty SPF-grade SD rats were selected and divided into control,model and experimental groups, in which the control group was not given treatment,the model and experimental groups were established as chronic periodontitis (CP) rat models,with 10 rats in every group and the experimental group received DPSC-Exos injections into the palatal gingiva of the bilateral maxillary first molars.The distance from the enamel bone boundary to the top of the alveolar ridge (CEJ-ABC) and periodontal tissue changes were compared among the three groups of rats.Periodontal ligament stem cells (PDLSCs) were selected and divided into a control group and an experimental group,no treatment was given to the control group,and DPSC-Exos was added to the petri dish of the experimental group,to compare the migration ability,proliferation ability,and the number of calcaneal nodules of the two groups,and to detect the expression of Runt-related transcription factor-2 (Runx-2) mRNA,Bone Salivary Protein (BSP) mRNA,and protein expression of the cells in the two groups. Results No abnormalities were observed in the periodontal tissues of rats in the control group;in the periodontal tissues of rats in the model group,there were disorders in the arrangement of collagen fibres,a large number of inflammatory cell infiltration,obvious proliferation of epithelium in the pocket with a shift towards the root,and resorption of the alveolar bone;in the periodontal tissues of the experimental group,there was a reduction in the infiltration of inflammatory cells compared with that of the control group,and the epithelium of the gingival sulcus did not have obvious proliferation and shift towards the root.The CEJ-ASC distance of rats in the model group was significantly higher than that of the control group; the CEJ-ASC distance of rats in the experimental group was significantly lower than that of the model group (P＜0.05).The OD value of the cells in the experimental group was significantly higher compared with the control group in the 12h,24h and 72h time points;the migration rate of the cells in the experimental group was significantly higher compared with the control group in the 12h and 24h time points (P＜0.05).The number of cellular calcium nodules in the experimental group was significantly higher compared with the control group (P＜0.05).The number of cellular Runx-2 mRNA,BSP mRNA,Runx-2 protein,and BSP protein was significantly higher in the experimental group compared with the control group (P＜0.05). Conclusion DPSC-Exos can reduce inflammatory symptoms and decrease the degree of bone resorption in periodontitis rats, while DPSC-Exos can promote the proliferation and migration activities of PDLSCs cells,and enhance the osteogenic differentiation ability of PDLSCs cells,the mechanism of which may be related to its mediation of Runx-2 and BSP expression. |
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