| 赵颖,孙崇然,王宇飞,张莹,王晓丽,朱友双.粗毛栓菌原生质体UV诱变漆酶高产菌株的选育[J].济宁医学院学报,2015,(2):106-109 |
| 粗毛栓菌原生质体UV诱变漆酶高产菌株的选育 |
| UV-Mutagenesis and screening of high laccase-producting strains from trametes gallica protoplasts |
| 投稿时间：2015-03-29 |
| DOI：10.3969/j.issn.1000-9760.2015.02.008 |
| 中文关键词: 漆酶 粗毛拴菌 原生质体 紫外诱变 |
| 英文关键词: Laccase Trametes hirsuta Protoplast UV mutagenesis |
| 基金项目:山东省自然科学基金联合专项(编号:ZR2014CL002);2014山东省高等学校青年骨干教师国内访问学者项目;济宁医学院青年科研基金资助项目(编号:JYQ2011KM012);济宁医学院博士科研启动基金(编号:094201) |
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| 中文摘要: |
| 目的 以粗毛栓菌Trametes hirsuta lg-9为出发菌株进行原生质体的UV诱变,以期获得漆酶高产菌株。方法 优化粗毛栓菌Trametes hirsuta lg-9原生质体的制备方法,并摸索该菌株紫外诱变条件。结果 筛选出2株漆酶高产菌株JM-70S3和JM-70S8,分别是出发菌株Trametes hirsuta lg-9漆酶产量的93.57倍和257.24倍。结论 原生质体紫外诱变是粗毛拴菌获得漆酶高产菌株的有效方法。 |
| 英文摘要: |
| Objective To obtain the high laccase-producing strains, we used the method of UV-mutagenesis to the Trametes hirsuta lg-9 protoplasts.Methods We optimized the protoplast preparation method of bacteria Trametes hirsuta lg-9, and groped for the strain ultraviolet mutagenesis condition.Results JM-70S3 and JM-70S8 were screened out, which produced laccase 93.57 and 257.24 higher than the original strains.Conclusion The UV-mutagenesis is an effective method to obtain the high laccase-producing strains of Trametes hirsuta. |
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