| 张胜波,屈胜环.微生物比浊法测定硫酸庆大霉素颗粒效价[J].济宁医学院学报,2013,(4):259-260 |
| 微生物比浊法测定硫酸庆大霉素颗粒效价 |
| Determination of the potency of Gentamicin Sulfate Granules by turbidimetric method and analysis |
| 投稿时间：2013-04-25 |
| DOI：10.3969/j.issn.1000-9760.2013.04.008 |
| 中文关键词: 比浊法 硫酸庆大霉素颗粒 效价 |
| 英文关键词: Sturbidimetric method, Gentamicin sulfate granules, Potency |
| 基金项目: |
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| 中文摘要: |
| 目的 建立微生物比浊法测定硫酸庆大霉素颗粒效价的方法。方法 以金黄色葡萄球菌为试验菌,菌液浓度为1.0%,培养测定时间为3~4h。结果 硫酸庆大霉素浓度在0.2~1.0U/ml的范围内,浓度的对数与吸光度成良好的线性关系:A=0.6214-0.7187lgC (r=0.9989),平均回收率为99.2%,RSD为0.25%。结论 微生物比浊法测定硫酸庆大霉素颗粒的效价简便、快速。 |
| 英文摘要: |
| Objective Establish a turbidimetric method for determination the content of Gentamicin Sulfate Granules.Methods The test organism was staphylococcus aureus, adjusted spore suspension of staphylococcus aureus inoculating with a 1.0% ratio, cultured at 37℃ for about 3~4h.Results The calibration curve was liner in the range of 0.2~1.0U/mL, while the calibration curve linear equation is A=0.6214-0.7187lgC(r=0.9989), and the average recovery is 99.2% with RSD of 0.25%.Gentamicin sulfate in concentration in 0.2~1.0U/mL, within the scope of the concentration of the logarithm and absorbency into good linear relationship between the linear equation:A=0.6214-0.7187lgC(r=0.9989), and the average recovery is 99.2%, and RSD is 0.25%.Conclusion Microbial turbidity method for the determination of Gentamicin Sulfate Granules titer is simple, accurate and fast. |
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