| 张华丽,朱军,高桂花.山东肿足蕨高效液相色谱指纹图谱初步研究[J].济宁医学院学报,2011,(6):389-391 |
| 山东肿足蕨高效液相色谱指纹图谱初步研究 |
| HPLC Fingerprint Spectrum of Hypodematium sinense lwatsuki |
| 投稿时间：2011-11-15 |
| DOI：10.3969/j.issn.1000-9760.2011.06.003 |
| 中文关键词: 山东肿足蕨|高效液相色谱法|指纹图谱 |
| 英文关键词: Hypodematium Sinense Iwatsuri|HPLC|Fingerprint Spectrum |
| 基金项目:济宁医学院2008年青年科研基金项目 |
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| 中文摘要: |
| 目的 建立山东肿足蕨药材的高效液相色谱指纹图谱,为其质量控制提供参考。方法 采用高效液相色谱(HPLC)法,色谱柱VP-ODS,流速1ml/min,检测波长215nm,柱温25℃,用乙腈和水作为流动相,梯度洗脱,测定了10批山东肿足蕨样品。结果 得到了山东肿足蕨HPLC指纹图谱。结论 用文中的最佳条件可较全面地反映山东肿足蕨中的成分,为山东肿足蕨药材的质量控制提供参考。 |
| 英文摘要: |
| Objective HPLC fingerprint spectrum of Hypodematium Sinense Iwatsuri was established in order to provide reference for its quality control. Methods RP-HPLC(DAD) was used in the determination of Hypodematium Sinense Iwatsuri by gradient elution under the following condition:column of VP-ODS, flow rate of 1mL/min, detection wavelength of 215 nm, temperature of 25℃ and acetonitrile and water as mobile phase.10 batches of Hypodematium Sinense Iwatsuri were determined. Results A preferable method for HPLC fingerprint determination of the Hypodematium Sinense Iwatsuri was established. Conclusion A general acquaintance of the Hypodematium Sinense Iwatsuri can be obtained by using the preferable HPLC fingerprint determination method, which is useful for quality valuation of the Hypodematium Sinense Iwatsuri. |
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