| 刘君,全先高,马蒙,石振琛.蜂胶总黄酮的提取及其紫外光度法测定[J].济宁医学院学报,2011,(5):321-322,353 |
| 蜂胶总黄酮的提取及其紫外光度法测定 |
| Study on the extract of total flavones in propolis |
| 投稿时间：2011-09-15 |
| DOI：10.3969/j.issn.1000-9760.2010.05.006 |
| 中文关键词: 蜂胶 总黄酮 提取 紫外分光光度法 芦丁 |
| 英文关键词: Propolis Total flavones Extraction UV Rutin |
| 基金项目:济宁市科技局立项项目 |
|
| 摘要点击次数: 6234 |
| 全文下载次数: 1988 |
| 中文摘要: |
| 目的 探讨不同提取方法对测定蜂胶中总黄酮含量的影响。方法 在502nm处,以芦丁为标准品,用紫外分光光度法测定不同提取方法所提取的蜂胶中总黄酮的含量。结果 测得工作曲线的回归方程为A=0.01149C+0.00772,线性相关系数R=0.9997,表明芦丁浓度在8μg/ml~50μg/ml范围内,与吸光度呈良好的线性关系。精密度RSD%=0.02(n=6),回收率为99.92%(n=4)。结论 超声提取法的提取率要比甲醇索氏提取法的提取率高,且方法简便易行。 |
| 英文摘要: |
| Objective To explore the effect of different extraction methods on the content of total flavones from propolis.Methods Using Rutin as standard material, a UV determination method was studied the content of total flavones extracted from propolis at 502nm.Results The linear regression equation of the UV determination method is A=0.01149C+0.00772.The precision(RSD%, n=6) is 0.02 and the recovery is 99.92%(n=4).Conclusion The ultrasonic extraction method is better than methanol Soxhlet extraction method.The ultrasonic extraction method was simple, effective and practical, and could be used to determine the content of total flavones from propolis when necessary. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
