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| 蒲公英总黄酮含量测定及对小鼠体液免疫功能影响的研究* |
| Determination of total flavonoids content and effects of Taraxacum mongolicum Hand.Mazz. on humoral immune function in mice |
| 投稿时间：2017-04-18 修订日期：2018-04-30 |
| DOI： |
| 中文关键词: 蒲公英；总黄酮；含量测定；体液免疫 |
| 英文关键词: Taraxacum mongolicum Hand.Mazz.; Total flavonoids; Content determination; Humoral immunity |
| 基金项目: |
| 作者 | 单位 | 邮编 | | 李艳芝* | 济宁医学院药学院 | 276826 | | 张千 | 济宁市食品药品检验检测中心 | | | 刘树玲 | 济宁医学院药学院 | | | 刘玉国 | 济宁医学院药学院 | |
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| 中文摘要: |
| 目的 研究不同时期蒲公英（根、叶、花）总黄酮含量变化规律及含量最高时期蒲公英根、叶、花总黄酮对小鼠体液免疫功能的影响。方法 超声提取法提取蒲公英中总黄酮，用HPD-400型大孔树脂进行纯化，紫外分光光度法测定总黄酮含量；溶血素测定法测定蒲公英总黄酮体液免疫功能。 结果 蒲公英总黄酮含量4月10日最高。同一时期蒲公英根、叶、花中总黄酮含量高低为花>叶>根。蒲公英根、叶、花总黄酮均有增强小鼠体液免疫的作用，其中花作用最强，与黄芪精口服液相比，差异无统计学意义（P ＞0.05）。 结论 不同时期蒲公英根、叶、花中总黄酮含量不同，4月10日蒲公英花含量最高；蒲公英花总黄酮增强小鼠体液免疫的作用最强。 |
| 英文摘要: |
| Objective To study the changes of total flavonoids in different stages of Taraxacum mongolicum Hand.Mazz.(root, leaf, flower) and the effects of total flavonoids on the humoral immune function in mice. Methords Total flavonoid was extracted by ultrasonic extraction method and purified by HPD-400 macroporous resin. Ultraviolet spectrophotometric method was used for the determination of the content of total flavones of T. mongolicum Hand.Mazz. in different periods, hemolysin assay method was used for determination of humoral immune function. Results The content of total flavonoids of root, leaf and flower from T. mongolicum Hand.Mazz. were different in different periods. In the same period, the content of total flavonoids from high to low is flower>leaf>root. The total flavonoids of root, leaf and flower from T. mongolicum Hand.Mazz. could enhance humoral immune effect (compared with negative control group, P < 0.05). The total flavonoids of flower had the strongest effect in enhancing humoral immune function in mice, which compared with Astragalus essence oral liquid there was not statistically significant (P > 0.05). Conclusion The content of total flavonoids of flower from T. mongolicum Hand.Mazz. collected in April 10 was much higher than others. The total flavonoids of flower had the strongest effect in enhancing humoral immune function in mice. |
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