| 高文明,李嘉铮,王慧青,董康,孟尧,程葆华.Ghrelin对6-OHDA诱导SH-SY5Y细胞线粒体损伤和细胞凋亡的影响[J].济宁医学院学报,2021,44(3):179-183,188 |
| Ghrelin对6-OHDA诱导SH-SY5Y细胞线粒体损伤和细胞凋亡的影响 |
| Effects of ghrelin on 6-OHDA-induced mitochondrial famage and apoptosis in SH-SY5Y cells |
| 投稿时间：2021-03-29 |
| DOI：10.3969/j.issn.1000-9760.2021.03.007 |
| 中文关键词: Ghrelin;6-OHDA;帕金森病;线粒体损伤;凋亡 |
| 英文关键词: Ghrelin;6-OHDA;Parkinson's disease;Mitochondrial dysfunction;Apoptosis |
| 基金项目:济宁医学院大学生创新训练计划项目（S201910443006） |
| 作者 | 单位 | E-mail | | 高文明 | 济宁医学院基础医学院 | | | 李嘉铮 | 济宁医学院临床医学院, 济宁 272000 | | | 王慧青 | 山东大学齐鲁医学院, 济南, 250014 | | | 董康 | 济宁医学院临床医学院, 济宁 272000 | | | 孟尧 | 济宁医学院临床医学院, 济宁 272000 | | | 程葆华 | 济宁医学院基础医学院 | chengbh1979@163.com |
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| 中文摘要: |
| 目的 观察Ghrelin能否保护人神经母细胞癌细胞（SH-SY5Y细胞）抵抗6-OHDA所致的线粒体损伤和细胞凋亡。方法 培养SH-SY5Y细胞，随机分为对照组、Ghrelin组、6-OHDA组和6-OHDA+Ghrelin组。通过6-OHDA处理SH-SY5Y细胞构建帕金森病（Parkinson's Disease，PD）体外模型。Ghrelin预处理后，CCK-8法观测细胞活力，乳酸脱氢酶（Lactate dehydrogenase，LDH）法观察细胞毒力，JC-1染色观察线粒体膜电位（ΔΨm），BCL-2/Bax比例检测观察细胞凋亡。结果 与对照组相比，6-OHDA组细胞活力下降，细胞毒力增加，ΔΨm以及BCL-2/Bax比例下降（均P<0.05）；Ghrelin预处理后，与6-OHDA组相比，6-OHDA+Ghrelin组细胞活力上升，细胞毒力降低，ΔΨm以及Bcl-2/Bax比例增高（均P<0.05）。结论 Ghrelin预处理可以保护SH-SY5Y细胞抵抗6-OHDA所致细胞损伤，具有线粒体保护功能，减少细胞凋亡。 |
| 英文摘要: |
| Objective To observe whether Ghrelin can protect SH-SY5Y cells against mitochondrial damage and apoptosis caused by 6-OHDA.Methods SH-SY5Y cells were culutured and randomly divided into control group,Ghrelin group,6-OHDA group and 6-OHDA+Ghrelin group.We utilized human neuroblastoma SH-SY5Y cells exposed to 6-OHDA as a PD model in vitro.After pretreatment with Ghrelin,cell viability was measured by CCK-8,cytotoxicity was observed by LDH,and mitochondrial membrane potential (ΔΨm) by JC-1 staining.Additionally,the ratio of Bcl-2/Bax reflected the cell apoptosis.Results Compared with the control group,cell viability,ΔΨm,and the ratio of Bcl-2/Bax were decreased while the cytotoxicity was increased in the 6-OHDA group.(all P<0.05).Compared with the 6-OHDA group,pretreatment with Ghrelin increased cell vitality,ΔΨm,and the ratio of Bcl-2/Bax but decreased the cytotoxicity (all P<0.05).Conclusion Pretreatment with Ghrelin protected SH-SY5Y cells against cell damage,ameliorated the mitochondrial dysfunction,and reduced cell apoptosis induced by 6-OHDA. |
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