| 徐兴华,郭汶慧,王凯悦,任公卿,陈彤,王建礼.大蒜素对BALB/c小鼠溃疡性结肠炎的影响及其作用机制[J].济宁医学院学报,2020,43(5):326-329 |
| 大蒜素对BALB/c小鼠溃疡性结肠炎的影响及其作用机制 |
| The effect of allicin on ulcerative colitis in BALB/c and its mechanism |
| 投稿时间：2020-07-09 |
| DOI：10.3969/j.issn.1000-9760.2020.05.005 |
| 中文关键词: 大蒜素;溃疡性结肠炎;TNF-α;髓过氧化物酶 |
| 英文关键词: Allicin;Ulcerative colitis;TNF-α;MPO |
| 基金项目:山东省高等学校国家级大学生创新创业训练计划项目（201810443017）；济宁医学院大学生创新训练计划项目（cx2018021） |
| 作者 | 单位 | E-mail | | 徐兴华 | 济宁医学院基础医学院, 济宁 272067 | | | 郭汶慧 | 济宁医学院基础医学院, 济宁 272067 | | | 王凯悦 | 济宁医学院基础医学院, 济宁 272067 | | | 任公卿 | 济宁医学院基础医学院, 济宁 272067 | | | 陈彤 | 济宁医学院基础医学院, 济宁 272067 | | | 王建礼 | 济宁医学院基础医学院, 济宁 272067 | wangjianli2019@163.com, |
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| 中文摘要: |
| 目的 探讨大蒜素对小鼠溃疡性结肠炎（UC）的治疗作用及其相关作用机制。方法 BALB/c小鼠60只，雌雄各半，随机分为3组：正常组、模型组和大蒜素组，每组20只。正常组小鼠自由饮用蒸馏水，其余组小鼠自由饮用5%葡聚糖硫酸钠（DSS）溶液建立UC模型，建模开始即按分组灌胃给药，空白组和模型组给予生理盐水，大蒜素组给予等量大蒜素溶液（2g·L-1），给药期间观察小鼠体征变化，进行疾病活动指数（DAI）评估，连续7d，眼球取血后处死小鼠，采集结肠组织进行病理学检查。ELISA法测定小鼠血清样本中TNF-α水平；髓过氧化物酶（myeloperoxidase，MPO）活性检测试剂盒测定小鼠结肠组织中MPO活性。结果 与模型组相比，大蒜素组小鼠结肠长度较长，DAI分数较低（P<0.025）；血清中TNF-α含量降低（P<0.025）；结肠组织中MPO活性也明显下降（P<0.025）。结论 大蒜素对UC小鼠有治疗作用，其可能通过抑制TNF-α释放，下调促炎因子MPO活性，从而阻断炎症的放大效应，达到治疗效果。 |
| 英文摘要: |
| Objective To investigate the therapeutic effect of allicin on ulcerative colitis (UC) in mice and its mechanism.Methods 60 BALB/c mice were randomized into 3 groups:Normal,model and allicin group,20 in each group.Except the normal group,the mice in other groups drank 5% dextran sodium sulfate(DSS )aqueous solution freely to establish UC model.From the beginning of modeling,the mice in model group were given allicin solution(2g·L-1).The normal and model group were given the same amount of normal saline once a day for 7 consecutive days.The changes of physical signs of mice were observed,disease activity index (DAI) was evaluated,and the colon tissues of mice were examined by microscopy.The content of TNF-α in serum samples of mice was measured by ELISA,and the activity of MPO in colon tissues of mice was detected by MPO kit.Results Compared with the model group,the colon length of the allicin group was longer,the DAI score was lower (P<0.05),the serum TNF-α content was lower (P<0.05),and the activity of MPO in colon tissue was also significantly lower (P<0.05).Conclusion Allicin has therapeutic effect on UC mice.It may block the amplification effects of inflammation by inhibiting the release of TNF-α and down-regulating the activity of pro-inflammatory factor MPO. |
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