| 徐冰,张玉修,曹胜男,孙建楠.乌头类药材及其中成药乌头碱类化合物的含量测定[J].济宁医学院学报,2020,43(4):238-241,247 |
| 乌头类药材及其中成药乌头碱类化合物的含量测定 |
| Content assessment of aconitines compounds in Aconitum and related proprietary Chinese medicine |
| 投稿时间：2019-12-30 |
| DOI：10.3969/j.issn.1000-9760.2020.04.003 |
| 中文关键词: 高效液相色谱法;乌头碱类化合物;中成药 |
| 英文关键词: HPLC;Aconitine substance;Proprietary Chinese medicine |
| 基金项目:济宁医学院博士基金（JY2015BS05）；济宁医学院大学生创新创业项目（cx2019082） |
| 作者 | 单位 | E-mail | | 徐冰 | 济宁医学院公共卫生学院, 济宁 272013 | | | 张玉修 | 济宁市中医院, 济宁 272027 | | | 曹胜男 | 济宁医学院公共卫生学院, 济宁 272013 | | | 孙建楠 | 济宁医学院公共卫生学院, 济宁 272013 | sunjn@mail.jnmc.edu.cn |
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| 摘要点击次数: 1959 |
| 全文下载次数: 934 |
| 中文摘要: |
| 目的 建立乌头类药材及其中成药乌头碱类化合物含量的测定方法，为中成药质量安全评价提供依据。方法 以单因素实验对影响超声提取法萃取效率的提取溶剂、超声时间和提取温度等因素进行优化。采用高效液相色谱法测定乌头类两种中药和三种中成药的乌头碱类化合物。结果 确定了超声提取法的最佳萃取条件。根据建立的高效液相色谱法，乌头碱和新乌头碱分别在3.8~60.8μg/ml和5.6~89.6μg/ml浓度范围内与峰面积呈现良好的线性相关（r乌头碱=0.9998；r新乌头碱=09994）；RSD值均小于5.21%，加标回收率在97.53%~103.28%。结论 所建立的方法准确可靠，将其分别用于市售川乌、附子、附子理中丸、桂附地黄丸和伤湿止痛膏中乌头碱和新乌头碱含量分析，乌头碱及新乌头碱含量处在安全范围内，符合药典标准。 |
| 英文摘要: |
| Objective To establish a method for simultaneous determination of aconitines compounds in Aconitum and related proprietary Chinese medicine,and to provide a method for safety evaluation of proprietary Chinese medicine. Methods The extraction solvent,ultrasonic time and extraction temperature were optimized by single factor experiment.High performance liquid chromatography (HPLC) was used to determine aconitine in two kinds of Traditional Chinese Medicines and three kinds of Chinese patent medicines. Results The optimum extraction conditions for ultrasonic extraction were determined.According to the established HPLC method,the aconitine and mesaconitine exhibited good linearities (raconitine=0.9998;rmesaconitine=0.9994) in the concentration range of 3.8~60.8μg/ml and 5.6~89.6μg/ml,separately.The values of RSD were both lower than 5.21%,and the spiked recoveries were in the range of 97.53%~103.28%. Conclusion The established method is proved to be accurate and reliable.And this method has been used to evaluate the quantities of aconitine and mesaconitine in common Traditional and proprietary Chinese medicine including Radix Aconiti,Aconitum Carmichaeli Debx,Fuzi Lizhong pills,Guifu Dihuang pills and Shangshi Zhitong plaster.The results indicate that the contents of aconitine and mesaconitine are in the safety range,which also meet the standard of Chinese Pharmacopoeia 2015. |
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