| 刘乙,马瑜,殷澳,聂其婷,任强.绵马贯众薄层色谱指纹图谱的研究[J].济宁医学院学报,2020,43(1):10-13 |
| 绵马贯众薄层色谱指纹图谱的研究 |
| Study on TLC fingerprint of Dryopteris crassirhizoma in different drugstores |
| 投稿时间：2019-10-19 |
| DOI：10.3969/j.issn.1000-9760.2020.01.003 |
| 中文关键词: 绵马贯众;TLC;DPPH;FRAP |
| 英文关键词: Dryopteris crassirhizoma Nakai;Thin layer chromatography;2,2-diphenyl-1-picryl-hydrazyl;Ferric reducing antioxidant power |
| 基金项目:济宁医学院大学生创新创业训练计划资助项目（cx2015060）；国家级大学生创新创业训练计划资助项目（201510443004） |
| 作者 | 单位 | E-mail | | 刘乙 | 济宁医学院药学院, 日照 276826 | | | 马瑜 | 济宁医学院药学院, 日照 276826 | | | 殷澳 | 济宁医学院药学院, 日照 276826 | | | 聂其婷 | 济宁医学院药学院, 日照 276826 | | | 任强 | 济宁医学院药学院, 日照 276826 | renqiang@mail.edu.cn |
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| 中文摘要: |
| 目的 建立薄层色谱指纹图谱和抗氧化活性筛选对绵马贯众进行定性分析及其质量评价。方法 通过对来自19家药店绵马贯众的形状、大小、颜色、气味、切面、粉末等特征确定其真伪。将绵马贯众药材粉碎，加环己烷超声处理，离心后取上清液备用。薄层板经pH7.0的磷酸二氢钠-柠檬酸缓冲液加维生素C 1.2g摇匀浸渍改性后点样。展开剂正己烷-三氯甲烷-甲醇（30：15：1）展距9cm，建立薄层色谱指纹图谱。最后对其进行抗氧化实验，观察不同批次绵马贯众在抗氧化方面的差异。结果 性状鉴别、显微鉴别均显示所购药材均为绵马贯众真品，薄层色谱指纹图谱显示1~19号样品质量有较大差异，其中6、7、10号几乎无特征斑点，14号斑点颜色较浅。绵马贯众甲醇提取液对DPPH及FRAP的清除率显示，绵马贯众有较好的抗氧化活性。结论 不同药店购买的绵马贯众药品质量有较大差异，6、7和10号样品为不合格样品，可能受到产地、加工或存储时间的影响。 |
| 英文摘要: |
| Objective To establish a TLC fingerprint and antioxidant activity screening for qualitative analysis and quality evaluation of Dryopteris crassirhizomae.Methods Authenticity was determined by the shape,size,color,smell,section,and powder characteristics from 19 pharmacies of Dryopteris crassirhizoma.Dryopteris crassirhizoma crushed with cyclohexane ultrasonic treatment was centrifuged and the supernatant was spared.The sample was impregnated on the TLC plate modified by pH7.0 sodium di-hydrogen phosphate citric acid buffer plus vitamin C1.2 g.Developing agent was n-hexane,chloroform,methanol (30:15:1) and the spread distance was 9cm.ATLC fingerprint was established.Finally,Dryopteris crassirhizoma of different sources were observed through their difference antioxidant abilities.Results Macroscopic and microscopic identification showed that the purchase of Chinese medicinal materials were genuine Dryopteris crassirhizoma.TLC fingerprints showed that there was difference among the sample 1-19 quality,and sample 6,7 and 10 was almost no characteristic spots as well as sample 14 with lighter in color characteristic spots.The ethanol extract of Dryopteris crassirhizoma to remove DPPH and FRAP showed that it had good antioxidant activities.Conclusion There is a great difference in traditional Chinese medicine quality of different pharmacy sources Dryopteris crassirhizoma.The unqualified samples 6,7,and 10 might be influenced by production,processing or storage time. |
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