| 文洁,蒋胜华,王娴玮,山凤莲.肺腺癌患者Breg Treg与IL-10 IL-35的表达及相关性研究[J].济宁医学院学报,2019,42(5):363-367,371 |
| 肺腺癌患者Breg Treg与IL-10 IL-35的表达及相关性研究 |
| Expression and correlation of Breg Treg and IL-10 and IL-35 in lung adenocarcinoma patients |
| 投稿时间：2019-06-15 |
| DOI：10.3969/j.issn.1000-9760.2019.05.013 |
| 中文关键词: 调节性T细胞/Treg细胞;调节性B细胞/Breg细胞;IL-10;IL-35;肺腺癌 |
| 英文关键词: Regulatory T cells/Treg;Regulatory B cells/Breg;IL-10;IL-35;Lung adenocarcinoma |
| 基金项目:济宁医学院教师扶持基金课题（JYFC2018FKJ075）；济宁医学院附属医院"苗圃"科研课题面上项目（2017-MP-MS-013） |
| 作者 | 单位 | E-mail | | 文洁 | 济宁医学院临床医学院, 济宁 272013 | | | 蒋胜华 | 济宁医学院附属医院, 济宁 272029 | | | 王娴玮 | 济宁医学院附属医院, 济宁 272029 | | | 山凤莲 | 济宁医学院附属医院, 济宁 272029 | shanfenglian@126.com |
|
| 摘要点击次数: 3348 |
| 全文下载次数: 839 |
| 中文摘要: |
| 目的 经流式细胞仪分析肺腺癌患者外周血CD4+CD25hi Tregs及其亚群包括aTreg、non-Treg、rTreg以及CD19+CD24hi CD27hi Bregs水平，探讨两群细胞及相应细胞因子IL-10、IL-35间的表达关系及临床意义。方法 选20例EGFR阳性并采用厄洛替尼方案治疗的肺腺癌患者为病例组，选30例年龄和性别与其相匹配的健康体检者为对照组。用流式细胞仪检测CD4+CD25hi Tregs细胞及其亚群（rTreg、non-Treg、aTreg）、CD19+CD24hi CD27hi Bregs细胞水平，采用酶联免疫吸附反应检测IL-10及IL-35的表达水平，比较肺腺癌患者与健康人群Treg、Breg、IL-10及IL-35的表达水平，并行相关性分析。结果 病例组Treg和Breg水平显著高于对照组（P<0.05）；其中aTreg占CD4+ T淋巴细胞的比例较对照组升高（P<0.05）。病例组治疗后Treg及Breg水平均较治疗前降低，但无统计学差异。相关性分析提示肺腺癌患者外周血中Breg与Treg呈正相关（r=0.513），IL-10与Treg和Breg呈正相关（r=0.637；r=0.791）。IL-35与Treg和Breg呈正相关（r=0.496；r=0.553）。结论 肺腺癌患者体内存在Breg与Treg细胞及其相关细胞因子IL-10、IL-35表达异常，推测它们共同参与免疫失衡，促进肿瘤发展。临床上可通过动态监测上述指标表达水平，利于实时评估非小细胞肺癌患者的免疫状态。 |
| 英文摘要: |
| Objective Flow cytometry was used to analyze the levels of CD4+CD25hi Tregs and its subgroups including aTreg,non-Treg and rTreg and CD19+CD24hi CD27hi Bregs in peripheral blood of lung adenocarcinoma patients,and to explore the expression relationship and clinical significance between the two groups of cells and corresponding cytokines.Methods Twenty patients with lung adenocarcinoma who were EGFR positive and treated with erlotinib were selected as the case group,and 30 healthy subjects whose age and sex matched with the case group patients were selected as the control group.Flow cytometry was used to detect the levels of CD4+CD25hi Tregs cells and their subsets(rTreg,non-Treg,aTreg),and CD19+CD24hi CD27hi Bregs cells.Enzyme Linked Immune Sorbent Assay was used to detect the expression levels of IL-10 and IL-35.The expression levels of Treg,Breg,IL-10 and IL-35 were compared between lung adenocarcinoma patients and healthy people,and the parallel correlation analysis was carried out.Results The levels of Treg and Breg in the case group were significantly higher than those in the control group(P<0.05).The proportion of aTreg in CD4+ T lymphocytes was higher than that in the control group(P<0.05).The Treg and Breg cell levels in the case group before treatment were higher than those after treatment(P>0.05).The Treg and Breg levels of patients after targeted therapy were lower than those before treatment(P>0.05).Correlation analysis showed that Breg was positively correlated with Treg in peripheral blood of lung adenocarcinoma patients(r=0.513,P<0.05),and IL-10 was positively correlated with Treg and Breg(r=0.637,P<0.05;r=0.791,P<0.05).IL-35 was positively correlated with Treg and Breg(r=0.496,P<0.05;r=0.553,P<0.05).Conclusion Abnormal expression of Breg and Treg cells and their related cytokines IL-10 and IL-35 exist in lung adenocarcinoma patients.It is presumed that they jointly participate in immune imbalance and promote tumor development.Clinically,the immune status of patients with non-small cell lung cancer can be evaluated in real time by dynamically monitoring the expression level of the above indicators. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
