| 王翠玲,李开智,崔文.HPV E6/E7 mRNA检测对宫颈HSIL/SCC的诊断价值[J].济宁医学院学报,2018,41(6):400-402,406 |
| HPV E6/E7 mRNA检测对宫颈HSIL/SCC的诊断价值 |
| The diagnostic significance of detection of human papillomavirus E6/E7 mRNA in high-grade squamous intra-epithelial lesions and squamous cell carcinoma of the cervix |
| 投稿时间：2018-04-15 |
| DOI：10.3969/j.issn.1000-9760.2018.06.006 |
| 中文关键词: 人乳头状瘤病毒;E6/E7 mRNA;高级别鳞状上皮内病变;鳞状细胞癌 |
| 英文关键词: Human papilloma virus;E6/E7 mRNA detection;High-grade squamous Intra-epithelial lesions;Squamous cell carcinoma |
| 基金项目: |
| 作者 | 单位 | E-mail | | 王翠玲 | 济南大学山东省医学科学院医学与生命科学学院, 济南 250012
济宁医学院附属医院, 济宁 272029 | | | 李开智 | 济宁医学院附属医院, 济宁 272029 | | | 崔文 | 济宁医学院司法鉴定中心, 济宁 272067 | cuiwenmd@163.com |
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| 中文摘要: |
| 目的 探讨人乳头瘤样病毒（human papilloma virus，HPV） E6/E7 mRNA与宫颈病变的关系，评价HPV E6/E7 mRNA检测在宫颈高级别鳞状上皮内病变（high-grade squamous intraepithelial lesion，HSIL）及鳞状细胞癌（squamous cell carcinoma，SCC）的诊断价值。方法 采用回顾性分析研究，选取济宁医学院附属医院2016年7月至2017年12月收治宫颈疾病患者、均取活检且组织学诊断明确的病例115例为观察对象，所有患者均行HPVE6/E7 mRNA检测，对其结果进行分析。以组织学诊断为标准，观察对象分为：HSIL/SCC 42例、低级别鳞状上皮内病变（low-grade squamous intraepithelial lesion，LSIL）42例、正常组织（normal tissues，Normal）31例，先比较3组间HPV E6/E7 mRNA阳性率差异，后合并组别比较Normal/LSIL与HSIL/SCC之间HPV E6/E7 mRNA阳性率差异。结果 HSIL/SCC HPV E6/E7 mRNA检测阳性33例（阳性率78.57%），LSIL阳性17例（阳性率40.48%），Normal阳性8例（阳性率25.81%）。HSIL/SCC阳性率显著高于Normal/LSIL，差异有统计学意义。结论 HPV E6/E7 mRNA在HSIL/SCC中表达显著，可作为重要标志物，对宫颈上皮内病变筛查及分流。 |
| 英文摘要: |
| Objective To investigate the relationship between E6/E7 mRNA of human papilloma virus (HPV) and cervical lesions,and estimate the value of HPV detection in the diagnosis of HSIL and SCC of the cervix.Methods A retrospective analysis was used to select patients with cervical diseases admitted to the Affiliated Hospital of Jining Medical University from July 2016 to December 2017.All patients were undergone biopsy and accurate histological diagnosis,and tested for HPV E6/E7 mRNA and analyzed.According to histological diagnosis,the observation objects were divided into 3 groups:HSIL/SCC(n=42),LSIL(n=42),normal tissues(n=31).HPV E6/E7 mRNA positive rates among the three groups was compared.Then,the differences of HPV E6/E7 mRNA positive rate between Normal/LSIL and HSIL/SCC were compared after the merger group.Results HPV E6/E7 mRNA was positive in 33 cases of HSIL/SCC (positive rate:78.57%),17 cases of LSIL (positive rate:40.48%),and 8 cases of normal tissue (positive rate:25.81%).The positive rate of HSIL/SCC was significantly higher than that of normal/LSIL,and the difference was statistically significant.Conclusion In women with HSIL/SCC,E6/E7 mRNA expression is higher which is an important marker,and playing a role of screening and triage. |
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