| 张倩,郭利伟,刘亚静,张咏鹤,徐光华,黄艳梅.河南新乡地区汉族群体MMPs基因启动子区5个SNPs与CHF发病风险关联性研究[J].济宁医学院学报,2018,41(1):48-52 |
| 河南新乡地区汉族群体MMPs基因启动子区5个SNPs与CHF发病风险关联性研究 |
| Correlation of MMPs promoter region five single nucleotide polymorphisms with genetic susceptibility of CHF in Han population from Xinxiang city of Henan province |
| 投稿时间：2017-11-09 |
| DOI：10.3969/j.issn.1000-9760.2018.01.013 |
| 中文关键词: 慢性充血性心力衰竭;基质金属蛋白酶;单核苷酸多态性;限制性扩增片段长度多态性 |
| 英文关键词: Matrix metallproteinases;Chronic heart failure;Single nucleotide polymorphism;Polymerase chain reaction restriction fragment length polymorphism |
| 基金项目:河南省医学科技攻关计划项目（201403131）；河南省教育科学“十二五”规划立项重点课题（2013-JKGHB-0035）；新乡医学院大学生科研创新计划课题（2014-21） |
| 作者 | 单位 | E-mail | | 张倩 | 新乡医学院法医学院, 新乡 453003 | | | 郭利伟 | 新乡医学院法医学院, 新乡 453003 | | | 刘亚静 | 新乡医学院法医学院, 新乡 453003 | | | 张咏鹤 | 新乡医学院法医学院, 新乡 453003 | | | 徐光华 | 新乡医学院法医学院, 新乡 453003 | | | 黄艳梅 | 新乡医学院法医学院, 新乡 453003 | hym_cn@hotmail.com |
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| 中文摘要: |
| 目的 探讨河南新乡地区汉族群体基质金属蛋白酶基因启动子区rs2285053、rs11225395、rs225207、rs243865及rs11568818这5个单核苷酸多态性（SNPs）与心力衰竭（CHF）发病的相关性。方法 知情同意情况下，采集160例CHF患者（观察组）和186例健康体检者（对照组）外周静脉血提取基因组DNA，限制性扩增片段长度多态性方法对5个SNPs进行分型，运用SHEsis软件分析数据。结果 与对照组相比，MMPs基因rs2285053位点T等位基因的频率在观察组中显著降低（P<0.05）； rs11225395位点的T等位基因和TT基因型频率在观察组中也显著降低（P<0.05），OR=1.966[95%CI（1.236~3.129）]；而观察组中rs225207的G等位基因频率显著增加（P<0.05），OR=0.499[95%CI（0.320~3.799]；rs2285053、rs11225395、rs225207组成的单体型CCG在观察组中的频率增高（P<0.05）。结论 rs11225395和rs2285053位点的等位基因T有可能降低CHF的患病风险；而rs225207位点的等位基因G及单体型CCG可能会增加CHF的患病风险。 |
| 英文摘要: |
| Objective To assess the relationship between 5 SNPs including rs2285053,rs11225395,rs225207,rs243865 and rs11568818 at the promoter region of matrix metalloproteinases (MMPs)genes and the risk of chronic heart failure (CHF)in Han population from Xinxiang city of Henan province.Methods 5 SNPs sites of 160 CHF patients (observation group)and 186 healthyin dividuals (control group)were genotyped by PCR-RFLP methods.Then the data was analyzed by SHEsis software.Results The frequency of T allele of rs11225395 of patients in the observation group was significantly lower than that in the control group (P<0.05).The frequency of T allele and TT genotype of rs11225395 of patients in the observation group was lower than that in the control group (P<0.05)OR=1.96[95%CI (1.236~3.129)].The G allele of rs225207 of patients in the observation group was higher than that in the control group (P<0.05),OR=0.499[95%CI (0.320~3.799].The haplotype CCG consisting of rs2285053,rs11225395 and rs2285053 of patients in observation group was higher than those in the control group (P<0.05).Conclusion T allele of rs2285053 and rs11225395,G allele of rs225207 and the haplotype CCG may decrease the risk of CHF. |
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