| 王慧云,程雅芳,李嫚琪,于林萍,李昊阳.纳米银的绿色生物合成及其抗菌实验研究[J].济宁医学院学报,2017,40(4):229-233,239 |
| 纳米银的绿色生物合成及其抗菌实验研究 |
| The study on the Green synthesis of silver nanoparticles and their anti-bacterial activity |
| 投稿时间：2017-07-10 |
| DOI：10.3969/j.issn.1000-9760.2017.04.001 |
| 中文关键词: 蒲公英提取液;生物合成;纳米银;抑菌活性 |
| 英文关键词: Taraxacum leaf extract;Biosynthesis;Silver nanoparticles;Anti-bacterial |
| 基金项目: |
| 作者 | 单位 | | 王慧云 | 济宁医学院药学院, 日照 276826 | | 程雅芳 | 济宁医学院药学院, 日照 276826 | | 李嫚琪 | 济宁医学院药学院, 日照 276826 | | 于林萍 | 济宁医学院药学院, 日照 276826 | | 李昊阳 | 济宁医学院药学院, 日照 276826 |
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| 中文摘要: |
| 目的 利用蒲公英叶提取液合成纳米银，为纳米材料生物合成领域的研究提供依据。方法 利用UV-2450紫外分光光度计、Zetasizer Nano-ZS型激光粒度仪考察了蒲公英提取液、硝酸银浓度、温度、反应时间、pH等条件对纳米银的粒径、分散系数PDI及紫外光谱的最大吸收波长的影响，并通过透射电子显微镜（TEM）、红外光谱仪（FTIR）及zeta电位的测定，对纳米银的特性进行了表征。进一步考察了所制备纳米银对革兰氏菌的抑菌活性。结果 得到球形的纳米银，粒度范围为30~60nm；紫外光谱的最大吸收波长为（432±20）nm；zeta电位在（-25.0±10）mV，所得纳米银粒子对革兰氏阳性菌具有更好的抑菌效果。结论 利用蒲公英提取液可实现对纳米银的可控合成。 |
| 英文摘要: |
| Objective To obtain the silver nanoparticles using the biosynthesizing method with the Taraxacum leaf extract and provide the basis of the study of the biosynthesis field of nano material.Methods The influence of the concentration of silver nitrate,Taraxacum leaf extract,temperature,pH values on the particle size,PdI and maximum absorption wavelength of UV were studied using UV-vis spectra and Zetasizer Nano-ZS laser particle size analyzer.And then the characters of silver nanoparticles was also analyzed with fourier transform infrared spectroscopy (FTIR),transmission electron microscopy (TEM) and X-ray diffract ometer(MRD).Moreover,the antibacterial activity was confirmed by anti-bacterial activity against gram bacteria.Results Spherical shapes were observed for biosynthesized SNPs within the range of 30~60 nm.An intense surface plasmon resonance band at (432±20)nm in the UV-visible spectrum clearly revealed the formation of silver nanoparticles.Zeta potential was (-25.0±10)mV.And the obtained SNPs had good bacteriostatic effect on Gram positive bacteria.Conclusion The controllable synthesis of silver nanoparticle can be realized using the Taraxacum leaf extract. |
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