张淑艺,秦红亚,商溪溪,王晓莹,张虎,王春梅.miR-34c在大鼠局灶性脑缺血模型中的表达[J].济宁医学院学报,2017,40(1):72-76 |
miR-34c在大鼠局灶性脑缺血模型中的表达 |
The expression of miR-34c in rat model of focal cerebral ischemia |
投稿时间:2016-11-07 |
DOI:10.3969/j.issn.1000-9760.2017.01.018 |
中文关键词: miR-34c;大鼠局灶性脑缺血;靶基因 |
英文关键词: miR-34c;Middle cerebral artery occlusion;Target genes |
基金项目:国家自然青年基金(81501018) |
作者 | 单位 | E-mail | 张淑艺 | 济宁医学院, 济宁 272067 | | 秦红亚 | 济宁医学院, 济宁 272067 | | 商溪溪 | 济宁医学院, 济宁 272067 | | 王晓莹 | 济宁医学院, 济宁 272067 | | 张虎 | 济宁医学院, 济宁 272067 | | 王春梅 | 济宁医学院基础医学院, 济宁 272067 | wangchunmei410@163.com |
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中文摘要: |
目的 通过检测miR-34c及其靶基因在大鼠局灶性脑缺血模型中的表达变化,初步探讨miR-34c及其靶基因在大鼠局灶性脑缺血中的作用。方法 首先利用线栓法制备大鼠局灶性脑缺血模型;然后利用qRT-PCR检测miR-34c在大鼠脑缺血不同时间点的表达变化;利用生物信息学方法预测miR-34c的可能靶基因;再利用RT-PCR和qRT-PCR方法检测靶基因在大鼠脑缺血不同时间点的表达变化。结果 成功制备大鼠局灶性脑缺血模型;miR-34c在大鼠脑缺血模型中随着缺血时间的延长其表达呈下降趋势;靶基因Hspa1b、Cacna2d1、Sperping1、Cntn2在大鼠脑缺血模型中随着缺血时间的延长其表达呈明显上升趋势。结论 miR-34c通过靶基因在大鼠局灶性脑缺血损伤过程中起到重要的保护作用。 |
英文摘要: |
Objective To detect the expression of miR-34c in middle cerebral artery occlusion (MCAO) model of rats and to preliminarily explore the function of miR-34c and its target genes in MCAO.Methods MCAO model of rats were prepared using thread embolism method.The expression of miR-34c was detected in model of MCAO at different times using qRT-PCR.The targets of miR-34c were predicted using bioinformatics methods.RT-PCR and qRT-PCR were further used to evaluated the expression of targets in model of MCAO at different times.Results MCAO model of rat was successfully prepared.The expression of miR-34c was gradually decreased in model of MCAO at different times.However,predicated targets were obviously up-regulated in model of MCAO at different times.Conclusion miR-34c and its targets are involved in the process of MCAO which play an important protective role. |
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