| 张兆强,张鲁华,林立,张春芝.矽尘对实验小鼠肺组织TOLL样受体2、4、9 mRNA表达的影响[J].济宁医学院学报,2017,40(1):21-24 |
| 矽尘对实验小鼠肺组织TOLL样受体2、4、9 mRNA表达的影响 |
| Effects of silica dust on expression of TLR2,4,9 mRNA in lung tissue of mice |
| 投稿时间：2016-08-09 |
| DOI：10.3969/j.issn.1000-9760.2017.01.005 |
| 中文关键词: 矽尘;肺纤维化;TLR2;TLR4;TLR9 |
| 英文关键词: Silica dust;Lung fibrosis;TLR2;TLR4;TLR9 |
| 基金项目:山东省卫生与计划生育委员会（2014WS0510）；济宁医学院科研计划项目（JY2015KJ003） |
| 作者 | 单位 | | 张兆强 | 济宁医学院公共卫生学院, 济宁 272067 | | 张鲁华 | 济宁医学院附属济宁市第一人民医院, 济宁 272011 | | 林立 | 济宁医学院公共卫生学院, 济宁 272067 | | 张春芝 | 济宁医学院公共卫生学院, 济宁 272067 |
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| 中文摘要: |
| 目的 探讨矽尘对实验小鼠肺组织TOLL样受体2、4、9（TLR2、4、9）mRNA表达的影响。方法 将40只小鼠随机分成对照组（不染尘）、2h组（每天染尘2 h）、4h组（每天染尘4 h）和8h组（每天染尘8 h），每组10只。20 d后，处死动物，取肺组织，提取总RNA、实时荧光定量PCR（RT-qPCR）检测不同暴露时间组TLR2、4、9 mRNA的相对含量。结果 对照组与3个暴露时间组TLR2 mRNA 的相对含量分别为：（0.98±0.69）、（1.18±0.85）、（2.56±0.54）、（3.22±0.77），4组间的差异有统计学意义（F=13.70，P=0.00）；与对照组相比，4h、8h组的表达升高，差异有统计学意义（P< 0.05）。对照组与3个暴露时间组TLR4 mRNA 的相对含量分别为：（0.93±0.32）、（1.16±1.23）、（4.80±5.31）、（7.00±6.22），4组间的差异有统计学意义（F=5.06，P=0.00）；与对照组相比，4h、8h组TLR4 mRNA的表达升高，差异统计学意义（P< 0.05）。对照组与3个暴露时间组TLR9 mRNA 的相对含量分别为：（1.04±0.25）、（1.29±0.30）、（1.26±0.41）、（2.21±0.33），4组间的差异有统计学意义（F=15.82，P=0.00）；与对照组相比，仅8h组的TLR9 mRNA 表达升高，差异有统计学意义（P< 0.05）。结论 染矽尘后，小鼠肺组织TLR2、4、9 mRNA的表达上调，这可能是启动肺纤维化的信号转导的起始步骤。 |
| 英文摘要: |
| Objective To study the expression of TLR2,4,9 mRNA of lung in mice exposed to silica dust.Methods 40 mice were randomly divided into 4 groups including control group,2h group (exposed to silica dust for 2 h every day),4h group (exposed to silica dust for 4 h every day) and 8h group (exposed to silica dust for 8 h every day).20 days later,all the lung of mice were taken to measure the expression of TLR2,4,9 mRNA by Realtime fluorescence quantitative PCR (RT-qPCR) technology.Results The relative contents of TLR2 mRNA of the control,2h,4h and 8h groups were (0.98±0.69),(1.18±0.85),(2.56±0.54) and (3.22±0.77).There were significantly difference among them (F=13.70,P=0.00).Compared with the control group,the expression of TLR2 mRNA of the 4h and 8h groups were significantly higher (P< 0.05).The relative contents of TLR4 mRNA of the control,2h,4h and 8h groups were (0.93±0.32),(1.16±1.23),(4.80±5.31) and (7.00±6.22).There were significantly difference among the 4 groups (F=5.06,P=0.00).Compared with the control group,the expression of TLR4 mRNA in the 4h and 8h groups were higher (P< 0.05).The relative contents of TLR9 mRNA of the control,2h,4h and 8h groups were (1.04±0.25),(1.29±0.30),(1.26±0.41) and (2.21±0.33).There were significantly difference among them (F=15.82,P=0.00).Compared with the control group,only the expression of TLR9 mRNA of the 8h groups were higher (P< 0.05).Conclusion The expression of TLR2,4,9 mRNA of the lung tissue of mice exposed to silica dust were up-regulated,which might be the initial step of the signal pathway leading to lung fibrosis. |
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