| 张兆强,林立,张春芝,聂继池.亚硝酸盐对实验小鼠的神经毒性作用[J].济宁医学院学报,2016,39(1):17-20 |
| 亚硝酸盐对实验小鼠的神经毒性作用 |
| The neurotoxic effects of nitrite on the experimental mice |
| 投稿时间：2015-09-03 |
| DOI：10.3969/j.issn.1000-9760.2016.01.004 |
| 中文关键词: 亚硝酸盐;行为功能;神经毒性;小鼠 |
| 英文关键词: Nitrite;Behavior function;Neurotoxicity;Mice |
| 基金项目: |
| 作者 | 单位 | | 张兆强 | 济宁医学院公共卫生学院, 济宁 272067 | | 林立 | 济宁医学院公共卫生学院, 济宁 272067 | | 张春芝 | 济宁医学院公共卫生学院, 济宁 272067 | | 聂继池 | 济宁医学院公共卫生学院, 济宁 272067 |
|
| 摘要点击次数: 7276 |
| 全文下载次数: 3449 |
| 中文摘要: |
| 目的 探讨经消化道亚硝酸盐染毒对实验小鼠产生的神经毒性作用。方法 将80只小鼠随机分为对照组、低剂量、中剂量和高剂量组, 每组20只。对照组给予自来水, 低剂量、中剂量、高剂量组分别自由饮浓度为0.055mg/ml、0.11mg/ml、0.22mg/ml的亚硝酸钠溶液。2周后, 分别进行小鼠后肢撑力、饥饿觅食、走格、鼠尾悬挂测试等反映神经毒性的系列实验。结果 小鼠后肢落地间距, 中剂量、高剂量组明显低于对照组(P<0.05), 与低剂量组比, 中、高剂量组的后肢落地间距均变低, 差异有统计学意义(P<0.05), 而中、高剂量组间无统计学意义(P>0.05);饥饿觅食试验第1次成功觅食时间, 各剂量组均明显比对照组时间长(P<0.05), 第2次成功觅食时间, 中剂量、高剂量组长于对照组(P<0.05), 且2个成功觅食时间都有随剂量增加时间更长的趋势;穿格次数, 高剂量组显著高于对照组(P<0.05), 其他组间的差异无统计学意义(P>0.05);鼠尾悬挂实验挣扎时间则表现为低剂量、中剂量、高剂量组比对照组短(P<0.05), 与低剂量组比, 中剂量组无统计学差异(P>0.05), 高剂量组则有统计学差异(P<0.05);高剂量组与中剂量组也有统计学差异(P<0.05), 且剂量越大, 挣扎时间越短。结论 亚硝酸盐对实验小鼠具有神经毒性。剂量越大, 毒性越明显。 |
| 英文摘要: |
| Objective To study the neutoxic effects of nitrite on the experimental mice.Methods 80 mice were randomly divided into control group, low dose group, middle dose group and high dose group.The control group was fed with tap water, and the low, middle, high dose group were fed with different concentration sodium nitrite (0.055mg/ml, 0.11mg/ml, 0.22mg/ml).2 weeks later, all the mice were measured with after-arm supporting force, hunger for food experiment, go grid, mouse tail suspension test.Results In the after-arm supporting force test, compared with the control group, the average length of middle dose and high dose group were significantly low (P<0.05).Compared with the low dose group, the average length of after arm supporting force of middle dose and high dose group were significantly lower(P<0.05), but there was no significant difference between middle dose and high dose groups (P>0.05).In the hungry for food experiment, the 1st successful foraging time of 3 experimental groups were all longer than the control group (P<0.05), and the 2nd successful foraging time of middle and high dose group were longer than the control group (P<0.05).Each of the 2 successful foraging time showed an increasing trend with the increasing dose (P<0.01).In go grid the time of high group was significantly higher than that of the control group (P<0.05), and there was no significant difference between the other groups (P>0.05).In the mouse tail suspension test, compared with the control group, the struggling time of 3 dose groups were shorter (P<0.05).Compared with the low dose group, the middle dose group had no significant difference, while the high dose group had statistical differences.And there was significant difference between high dose group and middle dose group.The struggle became shorter with the increasing dose.Conclusion Nitrite exposure had a neurotoxicity effects on the experimental mice.The higher the dose, the more obvious the toxicity. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
