| 白波,王春梅.利用RNA-Sequencing鉴定大鼠脑缺血-再灌注损伤模型中差异表达的基因[J].济宁医学院学报,2016,39(1):1-5,11 |
| 利用RNA-Sequencing鉴定大鼠脑缺血-再灌注损伤模型中差异表达的基因 |
| Identification of differentially expressed genes in rat model of cerebral ischemia-reperfusion injury by RNA-Sequencing |
| 投稿时间：2015-01-06 |
| DOI：10.3969/j.issn.1000-9760.2016.01.001 |
| 中文关键词: RNA-Sequencing;缺血-再灌注损伤;基因表达 |
| 英文关键词: RNA-Sequencing;Ischemia-reperfusion injury;Gene expression |
| 基金项目:山东省自然科学基金 (ZR2013CQ031);国家自然青年基金(81501018);济宁医学院博士启动基金(094301) |
| 作者 | 单位 | | 白波 | 济宁医学院神经生物研究所, 济宁 272067 | | 王春梅 | 济宁医学院神经生物研究所, 济宁 272067 |
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| 中文摘要: |
| 目的 利用RNA-Sequencing方法, 试图鉴定大鼠脑缺血-再灌注损伤模型中差异表达的基因, 为探讨脑缺血-再灌注损伤的分子机制提供实验依据。方法 首先利用RNA-Sequencing方法初步鉴定大鼠脑缺血-再灌注损伤模型中差异表达的基因, 然后利用生物信息学方法对差异表达基因的功能进行预测分析;再利用RT-PCR和qRT-PCR方法进一步检测差异基因的表达变化。结果 与对照组相比, 脑缺血-再灌注损伤实验组共鉴定出182个基因差异表达, 其中156个基因表达上调, 26个基因表达下调;RT-PCR和qRT-PCR方法进一步验证其中3个基因表达上调, 2个基因表达下调。结论 利用RNA-Sequencing方法可以鉴定大鼠脑缺血-再灌注损伤模型中差异表达的基因, 表明这些基因可能参与了脑缺血-再灌注损伤, 为进一步揭示脑缺血-再灌注损伤机制奠定了实验基础。 |
| 英文摘要: |
| Objective To identify differentially expressed genes in rat model of cerebral ischemia reperfusion injury using RNA-Sequencing and elaborate the molecular mechanism of cerebral ischemia reperfusion injury.Methods RNA-Sequencing was used to identify differentially expressed genes.Bioinformatics methods were used to predict the function of differentially expressed genes.RT-PCR and qRT-PCR were used to further detect the expression of different genes.Results Compared to control, 182 genes were identified differentially expressed in model of ischemia-reperfusion injury.156 genes were up-regulated and 26 genes were down-regulated.The expression of three genes were further proved to increase and two genes decrease using RT-PCR and qRT-PCR.Conclusion RNA-Sequencing could identify differentially expressed genes in rat model of cerebral ischemia-reperfusion injury suggesting that these genes may participate in cerebral ischemia-reperfusion injury.This study provides a basis to further reveal the mechanism of cerebral ischemia-reperfusion injury. |
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