| 徐蕴,窦珊珊,柳新平,崔利德,徐旭东.IL-6对大鼠骨髓间充质干细胞VCAM-1表达的影响[J].济宁医学院学报,2014,(3):162-165,175 |
| IL-6对大鼠骨髓间充质干细胞VCAM-1表达的影响 |
| The effect of IL-6 on expression of VCAM-1 in rat marrow mesenchymal stem cells |
| 投稿时间：2014-04-22 |
| DOI：10.3969/j.issn.1000-9760.2014.03.003 |
| 中文关键词: 骨髓间充质干细胞;白介素-6;黏附分子 |
| 英文关键词: Done marrow mesenchymal stem cell;IL-6;Adhesion molecules |
| 基金项目:2013年济宁医学院青年科研重点项目(编号:JY2013KJ014) |
| 作者 | 单位 | | 徐蕴 | 济宁医学院基础学院, 山东济宁 272067 | | 窦珊珊 | 济宁医学院基础学院, 山东济宁 272067 | | 柳新平 | 济宁医学院基础学院, 山东济宁 272067 | | 崔利德 | 济宁医学院基础学院, 山东济宁 272067 | | 徐旭东 | 济宁医学院基础学院, 山东济宁 272067 |
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| 中文摘要: |
| 目的 探讨白介素6(IL-6)对大鼠骨髓间充质干细胞(rMSCs)血管细胞黏附分子(VCAM-1)表达的影响。方法 将培养的rMSCs分为两组,各组均设6个样本:一组用终浓度为10 ng/ml的IL-6刺激12 h,另一组不加细胞因子刺激;然后采用免疫细胞化学方法检测两组细胞VCAM-1蛋白的表达,采用免疫荧光直标法检测两组细胞表面VCAM-1抗原的表达率,RT-PCR法检测两组细胞VCAM-1 mRNA的表达。结果 免疫细胞化学染色显示,未刺激组细胞染色浅,呈淡棕黄色;IL-6刺激组细胞呈棕褐色。流式细胞仪检测显示,未刺激组VCAM-1的表达率为14.1%;IL-6刺激组VCAM-1的表达率为80.9%。RT-PCR显示,未刺激组VCAM-1 mRNA呈微弱表达;刺激组VCAM-1 mRNA的表达显著增强(P<0.01)。结论 IL-6能诱导rMSCs VCAM-1的表达显著增高,提示VCAM-1可能在MSCs替代治疗初始阶段发挥重要作用。 |
| 英文摘要: |
| Objective To study the effect of IL-6 on the expression of VCAM-1 in rat marrow mesenchymal stem cells(rMSCs).Methods rMSCs were divided into two groups: the cells of one group were treated with IL-6(10ng/ml) for 12h and the other were regarded as control.VCAM-1 protein was identified by using immunocytochemical methods,the antigen of VCAM-1 were detected by flow cytometry and their mRNA were measured with RT-PCR.Results Immunocytochemistry and RT-PCR showed that low levels of VCAM-1 were expressed in the rMSCs,and flow cytometry showed that the rate of VCAM-1 expression was 14.1% in control group,which was 92.8% after pretreatment with IL-6.Conclusion IL-6 induced a concentration-dependent increase in VCAM-1 expression on MSCs.So we can see that VCAM-1 possibly plays an important role in the process of MSCs replacement therapy. |
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