| 李淑玲,凌爱霞.表面活性剂协同提取苦瓜中总黄酮的研究[J].济宁医学院学报,2012,(5):325-327,343 |
| 表面活性剂协同提取苦瓜中总黄酮的研究 |
| Study on Extraction of Flavonoids from the Bitter Melon Assisted by Surfactant |
| 投稿时间：2012-09-11 |
| DOI：10.3969/j.issn.1000-9760.2012.05.005 |
| 中文关键词: 表面活性剂;黄酮;苦瓜;正交试验 |
| 英文关键词: Surfactant;Flavonoids;Bitter melon;Orthogonal experiment |
| 基金项目: |
| 作者 | 单位 | | 李淑玲 | 济宁医学院基础学院, 山东济宁 272067 | | 凌爱霞 | 济宁医学院基础学院, 山东济宁 272067 |
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| 中文摘要: |
| 目的 提高苦瓜中总黄酮提取效率,筛选出其最佳提取工艺。方法 以苦瓜为原料,选取非离子型表面活性剂用于协同提取苦瓜中的总黄酮,研究表面活性剂用量、提取温度、提取时间、乙醇体积分数等因素对其影响。采用L9(34)正交试验确定苦瓜总黄酮的最佳提取工艺条件。结果 Tween-20 对苦瓜中总黄酮的提取具有较好的协同作用。各因素对黄酮提取率影响大小依次是:表面活性剂用量>提取温度>乙醇体积分数>提取时间。结论 最佳提取工艺条件乙醇浓度70%,Tween-20用量0.05g,提取时间4h,提取温度70℃。 |
| 英文摘要: |
| Objective To improve the extracting efficiency of total flavonoids from bitter melon, and filter out the best extracting process.Methods Experiments were carried out to explore the extraction of flavonoids from the bitter melon.The effects of surfactant usage, extracting temperature, extracting duration, ethanol volume percentage on extraction effect of total flavonoids from bitter melon were discussed.L9(34) orthogonal experiment was used to obtain the optimum extraction conditions of total flavonoids.Results Tween-20 was suit to explore the extraction of flavonoids from the bitter melon.Various factors on extraction effect sizes was as following:surfactant usage > extraction temperature > ethanol volume percentage > extracting duration.Conclusion The optimum extraction conditions were obtained as ethanol concentration 70%, Tween-200.05g, extraction at 70℃ for 4 hours. |
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