| 李京,任振峰.椎管减压对大鼠内质网跨膜蛋白Ire1α与细胞凋亡的影响[J].济宁医学院学报,2012,(5):322-324 |
| 椎管减压对大鼠内质网跨膜蛋白Ire1α与细胞凋亡的影响 |
| Effects of vertebral canal decompression on Ire1α expression and apoptosis following spinal cord injury in the rats |
| 投稿时间：2012-08-21 |
| DOI：10.3969/j.issn.1000-9760.2012.05.004 |
| 中文关键词: 抑制物阻抗性酯酶1;减压;内质网应激;细胞凋亡 |
| 英文关键词: Ire1α;Decompression;Endoplasmic reticulum stress(ERS);Apoptosis |
| 基金项目: |
| 作者 | 单位 | | 李京 | 济宁医学院基础学院, 山东济宁 272067 | | 任振峰 | 济宁医学院基础学院, 山东济宁 272067 |
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| 中文摘要: |
| 目的 观察椎管减压对于大鼠脊髓内质网跨膜蛋白Ire1α与细胞凋亡表达变化的影响。方法 健康成年SD大鼠60只,随机分为:1)椎管减压组(模型组)40只,每个时相点10只,采用压迫装置制备椎管减压模型;2)对照组10只,行脊髓持续压迫;3)假手术组10只,只做椎板切除。模型组分别于脊髓受压0.5、3、6、12h后解除压迫。采用免疫组化、Tunel染色观察压迫段脊髓内Ire1α的表达变化及细胞凋亡情况。结果 细胞凋亡数与Ire1α阳性细胞数均于0.5h减压时开始增高,二者随减压时间的延迟而不断增加。假手术组与对照组、各减压组之间相比均有显著性差异(P<0.05)。结论 大鼠脊髓压迫性损伤,尽早进行椎管减压,能够减轻内质网应激反应,减少Ire1α的表达与细胞凋亡的发生,促进神经功能恢复。 |
| 英文摘要: |
| Objective To investigate the effects of vertebral canal decompression on Ire1α expression and apoptosis following spinal cord injury in the rats.Methods Sixty adult SD rats(250±20 g) were randomly divided into consistently compressive group(control group, n=10) decompressive groups(n=40) and shame-operation group(n=10).The spinal cord was compressed posteriorly at the level of the first lumbar vertebra with a custom-made screw for 0.5, 3, 6 and 12 hours respectively, followed by decompression by removal of the screw.The sections were detected by immunohistochemistry and TUNEL staining.Results TUNEL-and Ire1α-positive cells were increased at 0.5 hour after decompression, and gradually elevated with the delay of decompression.The number of Ire1α-positive neurons was significantly lower than that of the control group(P<0.05).Conclusion The results indicate that the dynamic changes of Ire1α and apoptosis were correlated to decompression.Early decompression could reduce Ire1α expression and relieve the apoptosis following secondary spinal cord injury. |
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