| 于淼,袁莉,蒋方剑.芹菜素对人食管癌Eca109细胞增殖侵袭的影响[J].济宁医学院学报,2019,42(5):341-344 |
| 芹菜素对人食管癌Eca109细胞增殖侵袭的影响 |
| Effect of apigenin on proliferation and invasion of human esophageal cancer eca109 cells |
| 投稿时间：2019-08-15 |
| DOI：10.3969/j.issn.1000-9760.2019.05.008 |
| 中文关键词: 芹菜素;食管癌;细胞增殖;Eca-109细胞;侵袭转移 |
| 英文关键词: Apigenin;Esophageal Cancer;Cell Proliferation;Eca-109 Cell;Invasion Transmissi |
| 基金项目:山东省医药卫生科技发展项目（2016WS0100） |
| 作者 | 单位 | | 于淼 | 菏泽医学专科学校药学与制药工程系, 菏泽 274000 | | 袁莉 | 菏泽医学专科学校药学与制药工程系, 菏泽 274000 | | 蒋方剑 | 菏泽医学专科学校药学与制药工程系, 菏泽 274000 |
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| 中文摘要: |
| 目的 探究芹菜素对人食管癌Eca-109细胞增殖及侵袭的影响。方法 体外培养食管癌Eca-109细胞，随机分为芹菜素低、中、高组和空白对照组，MTT法检测芹菜素对常规培养的食管癌Eca-109细胞的生长抑制情况；流式细胞仪Annexin V/PI双染色法检测细胞凋亡以及对细胞周期的影响；Transwell侵袭实验观察芹菜素对Eca-109细胞侵袭、运动能力的影响。结果 MTT法结果显示，各芹菜素组对Eca-109细胞的生长抑制作用随剂量增加逐渐增强，呈现剂量依赖性，在24、48、72h，低剂量组的抑制率分别为11.28%、19.45%、37.78%，中剂量组的抑制率分别为14.47%、29.49%、49.8%，高剂量组的抑制率分别为15.78%、31.21%、67.21%，对照组在各时间段无抑制情况，实验组与对照组比较差别具有统计学意义（P<0.001）。Transwell侵袭实验显示，48h后芹菜素低、中、高剂量组和空白对照组细胞穿膜数分别为（78.50±3.48）个、（55.50±4.41）个、（37.75±3.36）个和（90.75±5.32）个，各芹菜素组与空白对照组比较差别具有统计学意义（P<0.001），不同浓度芹菜素组间两两比较具有统计学意义（P<0.001）。结论 芹菜素对人食管癌109细胞生长有抑制作用，能明显抑制其侵袭和运动能力。 |
| 英文摘要: |
| Objective To investigate the effect of apigenin on proliferation and invasion of human esophageal cancer Eca-109 cells in vitro.Methods In vitro culture of Eca-109 cells from esophageal cancer,logarithmic cells were taken for experiments.The inhibitory effect of apigenin on the growth of Eca-109 cells cultured in vitro by MTT assay,FlowcytometryAnnexin V/PI double staining for cell apoptosis and its effect on cell cycle,Transwell Invasion Test to Observe the Effect of Apigenin on Invasion and Motility of Eca-109 Cells.Results In MTT results,the experimental groups(5mg·L-1、10mg·L-1、20mg·L-1) were treated with apigenin,inhibitory effect on Eca-109 cell growth gradually increased in a dose-dependent manner.The inhibition rates of low dose group were 11.28%,19.45% and 37.78% at 24,48 and 72 hours respectively,the inhibition rates of middle dose group were 14.47%,29.49% and 49.8%at 24,48 and 72 hours respectively.The inhibition rates of high dose group were 15.78%,31.21% and 67.21% at 24,48 and 72 hours respectively in a time-dependent manner.There was no inhibition in the control group at all time intervals.The difference between the experimental group and the control group was statistically significant(P<0.001).There was statistical significance between two groups of different concentration and different time(P<0.001).Transwell Invasion Experiments showed that the number of cell membrane penetration in the control group was 90.75±5.32.The number of cell membrane penetration in low dose group was 78.50±3.48.The number of cell membrane penetration in the medium dose group was 55.50±4.41.The number of cell membrane penetration in the high dose group was 37.75±3.36.The difference between the experimental group and the control group was statistically significant(P<0.001).There was statistical significance between two groups with different concentrations(P<0.001).Conclusion Apigenin can inhibit the growth of human esophageal cancer 109 cells and significantly inhibit its invasion and motor ability. |
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