| 刘亚楠,王娜,梁秋华,张艳芳,黄园园,于世鹏.TUG1对地塞米松诱导小鼠MC3T3-E1细胞凋亡的影响[J].济宁医学院学报,2019,42(3):153-157 |
| TUG1对地塞米松诱导小鼠MC3T3-E1细胞凋亡的影响 |
| Effect of TUG1 on dexamethasone-induced apoptosis in mouse MC3T3-E1 cells |
| 投稿时间：2019-03-18 |
| DOI：10.3969/j.issn.1000-9760.2019.03.001 |
| 中文关键词: 成骨细胞;细胞凋亡;糖皮质激素;长链非编码RNA;TUG1 |
| 英文关键词: Osteoblasts;Apoptosis;Glucocorticoid;Long non-coding RNA;TUG1 |
| 基金项目:山东省医药卫生科技发展计划项目（2016WS0168） |
| 作者 | 单位 | E-mail | | 刘亚楠 | 济宁医学院临床医学院, 济宁 272013 | | | 王娜 | 济宁医学院附属医院, 济宁 272029 | | | 梁秋华 | 济宁医学院附属医院, 济宁 272029 | | | 张艳芳 | 济宁医学院附属医院, 济宁 272029 | | | 黄园园 | 济宁医学院临床医学院, 济宁 272013 | | | 于世鹏 | 济宁医学院附属医院, 济宁 272029 | yushipeng@vip_sina.com |
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| 中文摘要: |
| 目的 探讨长链非编码RNA-TUG1对地塞米松诱导小鼠MC3T3-E1细胞凋亡的影响。方法 体外培养MC3T3-E1细胞，建立细胞凋亡模型，诱导凋亡24h后qPCR检测TUG1的表达。将重组质粒导入MC3T3-E1细胞，获得稳定转染的细胞株，实验分为对照组、地塞米松组、地塞米松+TUG1过表达质粒组、地塞米松+阴性质粒组，qPCR检测TUG1的表达，流式细胞术检测细胞凋亡情况，免疫荧光观察Bcl-2的表达，计算平均荧光强度。结果 细胞凋亡模型成功建立，荧光显微镜显示质粒转染MC3T3-E1细胞，qPCR验证质粒转染成功（P<0.05）；地塞米松+TUG1过表达质粒组细胞凋亡明显低于地塞米松组和地塞米松+阴性质粒组，Bcl-2的表达在地塞米松+TUG1过表达质粒组明显高于地塞米松组和地塞米松+阴性质粒组，差异具有统计学意义（P<0.05）。结论 TUG1可以通过上调Bcl-2的表达抑制地塞米松诱导的小鼠MC3T3-E1细胞凋亡，TUG1可能是糖皮质激素诱导的成骨细胞凋亡的保护性因素。 |
| 英文摘要: |
| Objective To investigate the effect of long non-coding RNA-TUG1 in dexamethasone-induced mice MC3T3-E1 cell apoptosis.Methods MC3T3-E1 cells were cultured in vitro to establish a model of apoptosis.After induction of apoptosis for 24h,qPCR was used to detect the expression of TUG1.The recombinant plasmid was introduced into MC3T3-E1 cells to obtain a stably transfected cell line.The experiment was divided into control group,dexamethasone group,dexamethasone+TUG1 overexpression plasmid group and dexamethasone+negative plasmid group.qPCR was used to detect the expression of TUG1,and the apoptosis was detected by flow cytometry.Bcl-2 expression was observed byimmunofluorescence,and the mean fluorescence intensity was calculated.Results The apoptosis model was successfully established.Fluorescence microscopy showed that plasmid transfected cells were correctly transfected with qPCR (P<0.05).Apoptosis of dexamethasone+TUG1 overexpressing plasmid group was significantly lower than that of dexamethasone group and dexamethasone+negative plasmid group.Meanwhile,the expression of Bcl-2 was significantly higher in the dexamethasone+TUG1 overexpression plasmid group than in the dexamethasone group and the dexamethasone+negative plasmid group (P<0.05).Conclusion TUG1 can inhibit the apoptosis of mouse MC3T3-E1 cells induced by dexamethasone by up-regulating the expression of Bcl-2.TUG1 may be a protective factor for glucocorticoid-induced osteoblast apoptosis. |
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